New mTRAP Kits Purify mRNA from Cells, Tissue, or Total RNA

Qbiogene has introduced three new kits for purifying mRNA directly from cells, tissues, or total RNA. Formulated to achieve higher yields than oligo dT-based purification techniques, mTRAP mRNA Purification Kits incorporate a patent-pending peptide nucleic acid (PNA) molecular probe that has an extremely high binding affinity for mRNA. As a result, mRNA isolation can be performed in buffers with lower salt concentrations, eliminating secondary structure that can make mRNA inaccessible for capture as well as non-specific bonds that can cause unwanted rRNA and protein contamination. The system also allows mRNA to be selectively eluted away from unwanted genomic DNA and makes mRNA with short poly A tails more available for capture.

mTRAP Kits are available in two sizes for purification directly from tissue or cells and in one size for the purification of mRNA from total RNA. The 24-prep mTRAP Midi Kit typically yields 10-20 µg of mRNA from 0.5-1.0 x 10⁷ cells or 50-200 mg tissue while the 6-prep mTRAP Maxi Kit typically yields 100-200 µg of mRNA from 0.5-2.0 x 10⁸ cells or 0.4-1.0 gram tissue. mRNA yields from the 12-prep mTRAP Total Kit are typically 10-15 µg from each 500 µg of total RNA. All three kits include all required plasticware and RNase-free solutions.