New Hot
Start PCR Enzyme
The Hot
Prime™ Hot Start polymerase is a new thermostable polymerase that
is suitable for all PCR applications requiring “hot start” conditions.
The Hot Prime™ Hot Start polymerase is a chemically modified form
of recombinant Taq DNA polymerase. The chemical addition of heat-labile
blocking groups to specific amino acid residues of the Hot Prime™
enzyme renders it inactive at room temperature. The enzyme is easily activated
by a15 min incubation at 95°C. Once activated, the enzyme is functionally
equivalent to Taq DNA polymerase. The Hot Prime™ polymerase is specially
designed for “hot start” PCR since it is inactive during PCR
set-up and prior to initial denaturation and cannot elongate primer-templates
during non-optimal annealing temperatures.
In specially
designed laboratory tests, which deliberately accentuate problems commonly
encountered in PCR (i.e. non-specific hybridization, primer-dimer formation)
Hot Prime™ Hot Start polymerase consistently outperforms Taq DNA
polymerase. In all cases tested, under the same conditions, the results
obtained with Hot Prime™ Hot Start polymerase were comparable with
a leading competitor’s “hot start” polymerase.
Applications
for Hot Prime™ Hot Start polymerase include:
- Hot start
PCR
- Advance
preparation of Master Mixes
- Multiplex
PCR
- Detection
of low copy number template
- Automated
PCR
- Amplification
of templates that are GC rich, or contain secondary structure
*Sold under
license from Hoffmann-La Roche, Inc
More
on the Hot Prime™ Hot Start polymerase
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