New Hot Start PCR Enzyme
The Hot Prime Hot Start polymerase is a new thermostable polymerase that is suitable for all PCR applications requiring hot start conditions. The Hot Prime Hot Start polymerase is a chemically modified form of recombinant Taq DNA polymerase. The chemical addition of heat-labile blocking groups to specific amino acid residues of the Hot Prime enzyme renders it inactive at room temperature. The enzyme is easily activated by a15 min incubation at 95°C. Once activated, the enzyme is functionally equivalent to Taq DNA polymerase. The Hot Prime polymerase is specially designed for hot start PCR since it is inactive during PCR set-up and prior to initial denaturation and cannot elongate primer-templates during non-optimal annealing temperatures.
In specially designed laboratory tests, which deliberately accentuate problems commonly encountered in PCR (i.e. non-specific hybridization, primer-dimer formation) Hot Prime Hot Start polymerase consistently outperforms Taq DNA polymerase. In all cases tested, under the same conditions, the results obtained with Hot Prime Hot Start polymerase were comparable with a leading competitors hot start polymerase.
Applications for Hot Prime Hot Start polymerase include:
*Sold under license from Hoffmann-La Roche, Inc