RCA-free Adenovirus Amplification Service
with BMAdE1™ Packaging Cell Line
The new BMAdE1™ cell line is an E1-complementing cell line that eliminates the occurrence of Replication Competent Adenoviruses (RCA). First-generation E1-deleted recombinant Adenoviruses (rAd) are typically propagated in complementing Human Embryonic Kidney HEK-293 cell line which stably expresses the E1 gene products (Graham et al., 1977). RCAs are known to occur after several passages of amplification in HEK-293 cells. RCAs emerge following a double crossover event between the homologous overlapping sequences in the rAd genome and the E1 genes from the 293 cells, resulting in the loss of transgene and its replacement by E1 (Lochmüller et al., 1994, Hehir et al., 1996, Zhu et al., 1999). The presence of RCAs in Adenovirus stocks could have deleterious effects to the cells when used in gene delivery experiments in vitro, could be harmful to animals used for in vivo studies and pose potential risks to patients by activating the immune system or by acting as helper viruses for the replication of attenuated viral vectors (Imler et al., 1995).
The BMAdE1™ cell line was engineered to express the E1 genes without overlapping sequences with the adenovirus backbone (such as AdenoVator™, AdEasy™), thus eliminating the risk of generating RCAs in viral stocks. The new BMAdE1™ cell line provides scientists involved in functional genomics studies, gene delivery and gene therapy research with better control over RCAs in their viral stocks.
Features
· No homologous sequences with adenoviral backbones
· No risk of occurrence of RCA contaminants
· Affordable alternative for RCA-free cultures
· Available under research and commercial licenses or through custom services
Services available with the BMAdE1™ cell line
· Recombinant Adenovirus construction
· Plaque purification
· Amplification
· Purification
· Titration
· RCA detection: PCR- and cell-based
