Control Adenoviruses That Are RCA-free, Available Exclusively From MP Biomedicals
Free of Replication Competent Adenovirus (RCA-free)
Ready-to-use recombinant Adenoviruses
Highly purified by Double Caesium Chloride Gradient
For immediate in vitro and in vivo applications
Lower cytotoxicity than standard recombinant adenoviruses
RCAs (Replication Competent Adenoviruses) could have deleterious effects on in vivo and in vitro applications by activating the immune system or by acting as helper viruses for the replication of the attenuated viral vector (Imler et al., 1995). Indeed, exacerbation of host inflammatory responses with significant tissue damage and pathogenicity has been assigned to RCA contaminations (Lochmller et al., 1994; Hehir et al., 1996; Zhu et al., 1999). Expensive screening tests may be required to detect the presence of RCAs among a population of replication-defective recombinant Adenovirus.
Therefore, Qbiogene, leading manufacturer of recombinant Adenoviruses and now a member of MP Biomedicals, offers RCA-free ready-to-use control Adenoviruses. These purified, titered, tested and ready-to-use viruses express a CMV driven reporter gene (LacZ or GFP) or no transgene (negative control). Our RCA-free control viruses are perfectly suited for in vitro and in vivo applications.
In addition, we offer custom-made recombinant Adenovirus RCA-free as well as a proprietary RCA screening test, which allows the detection of as low as 1 RCA per 3 x 1010 recombinant Adenoviruses meeting the current FDA's recommendation on the RCA limit.
Hehir et al. 1996. Molecular characterization of replication-competent variants of adenovirus vectors and genome modifications to prevent their occurrence. J. Virol. 70: 8459-8467.
Imler et al. 1995. Trans-complementation of E1-deleted adenovirus: A new vector to reduce the possibility of codissemination of wild-type and recombinant adenoviruses. Hum. Gen. Ther. 6: 711-721.
Lochmller et al. 1994. Emergence of early region 1-containing replication-competent adenovirus in stocks of replication-defective adenovirus recombinants (dE1 dE3) during multiple passages in 293A cells. Hum. Gene Ther. 5: 1485-1491.
Zhu et al. 1999. Characterization of replication-competent adenovirus isolates from large-scale production of a recombinant adenoviral vector.
Hum. Gene Ther. 10:113-121.
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For more information on our New Ready-made RCA-free Recombinant Adenoviruses, please email us at email@example.com or
at 1-888-362-5487 (North America) or 514-935-2200 (worldwide).