Gene delivery consists of introducing DNA or RNA into cells, tissues,
or organisms, in order to study regulation and function of genes
and proteins. The biggest physical hurdle that gene delivery technologies
have to overcome is the cell membrane, which is impermeable to charged
macromolecules such as DNA and RNA. Numerous different gene delivery
methods using either chemical, physical and biological pathways
have been used in recent years and are constantly being improved
upon. Viral mediated introduction, liposome-based, and receptor
mediated transfection reagents are the most widely used techniques
for the introduction of the desired gene into the target cells.
Gene therapy aims at treating both genetic (e.g. cancer, haemophilia)
and infectious diseases (e.g. AIDS) by introducing new genetic material
into selected cells. The major challenge is to deliver the gene
safely and efficiently into the desired cells. One of the most successful
ways of introducing the gene of interest into the appropriate cell
line uses recombinant adenovirus. Recombinant adenovirus can also
be used in vaccination by expressing a gene product that triggers
an immune response.
For more information regarding how Qbiogene's Adenovirus Technology
can help you, click here.