AdenoExpress™: Ready-to-use Recombinant Adenoviruses

Description
For a number of years, many researchers have been using recombinant adenovirus with great success. Its numerous advantages have made recombinant adenoviral vectors the preferred gene delivery tool for mammalian cells. However, generating a recombinant adenovirus may be time-consuming. In order to overcome this time constraint, Qbiogene Molecular Biology has introduced the AdenoExpress™ vectors.

AdenoExpress™ vectors are recombinant ready-to-use adenoviruses. They have all the advantages of recombinant adenovirus with the additional benefits of being ready-made (cloned, purified, tested and at high-titer). Qbiogene’s AdenoExpress™ vectors carry the adenovirus serotype 5 backbone, and are replication defective due to the E1 region deletion and in addition E3 being deleted to provide additional cloning space (E1/E3).

For your convenience, AdenoExpress™ vectors are available carrying reporter genes, biological response genes, or as a control vectors with no gene at all.

At Qbiogene, we are constantly developing new AdenoExpress™ vectors, so please check our website on a regular basis for the most current list.

Reporter AdenoExpress™ Vectors

The Reporter Adenoviral Vectors carry a reporter gene (LacZ or GFP) driven by either the CMV or the even stronger CMV5 promoter. These adenoviruses are designed to help researchers gain experience with viral technology or to optimize infection and transfection parameters, such as the MOI (multiplicity of infection), with specific cells. The LacZ gene product can be easily detected by simple colorimetric assay, whereas the GFP can be used in real-time assays in living cells. Both GFP or LacZ Reporter AdenoExpress™ vectors can be used to test your specific in vitro or in vivo model.

AdenoExpress™ Biological Response

These viral vectors are fully characterized adenoviruses, expressing specific human or mouse biological response genes. Expression of the cloned gene is driven by the CMV promoter.

Q-mate™ AdenoExpress™

Q-mate™ AdenoExpress™ regroup several recombinant Adenoviruses designed for use in combination with the Q-mate™ Inducible Expression System. For more information, please click here.

Ad5.SV40-CymR

Ad5.SV40-CymR is a recombinant Adenovirus expressing the CymR gene under the control of the constitutive SV40 promoter. CymR is a repressor molecule that regulates the expression of a gene by binding to its operator sequences (CuO). This virus is used in co-infection with another Adenovirus which expresses a transgene in conjunction with the Q-mate™ Inducible Expression System.

Ad5.CMV5(CuO)-LacZ

Ad5.CMV5(CuO)-LacZ is a recombinant Adenovirus designed for inducible LacZ expression under the control of the strong CMV5 promoter. This virus is a control Adenovirus used in experiments involving the Q-mate™ Inducible Expression System.

Ad5.CMV5(CuO)-GFP

Ad5.CMV5(CuO)-GFP is a recombinant Adenovirus designed for inducible GFP expression under the control of the strong CMV5 promoter. This virus is a control Adenovirus used in experiments involving the Q-mate™ Inducible Expression System.

AdenoExpress™ Controls

Fully characterized and ready-to-use, Null AdenoExpress™ vectors do not contain any expression gene. They are particularly useful as control vectors to assess the effect of a recombinant Adenovirus in a specific system. For instance, the Null CMV vector is an excellent control vector for any Biological Response or Reporter Gene experiment, as the viral backbone is identical to that of the AdenoExpress™ vector of interest. In addition, Wild-type Adenovirus serotype 5 and serotype 2 are also available.

Advantages

• No cloning required
• No purification required
• No testing required
• No amplification required
• Highly purified ready-to-use recombinant adenovirus
• Available in different titers
• Ready for immediate in vitro and in vivo applications

To obtain additional contact or ordering information, click here.