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QBI-HEK 293A Cells

Description
The QBI-HEK 293A cell line is an immortalized line of primary human embryonic kidney cells transformed by sheared human Ad5 DNA. QBI-HEK 293A cells, harboring the E1A and E1B region of the adenoviral genome, complement in trans the deletion of the E1 region in the recombinant adenovirus. Therefore, the QBI-HEK 293A cell line is an excellent packaging cell line for the generation of high titer recombinant adenovirus.

In addition, and contrary to other 293 cell lines, Qbiogene’s QBI-HEK 293A cell line is a superior sub-clone of HEK 293 cells and strongly adheres to plastic dishes. QBI-HEK 293A cells do not require any special plasticware as long as it is treated for cell culture.

Furthermore, the QBI-HEK 293A cell line is very reliable, performing extremely well in plaque assay and transfection experiments.

QBI-HEK 293A cells are available in Qbiogene’s four Adenoviral Vector Systems (AdenoVator™, AdEasy™, Transpose-Ad™ and Adeno-Quest™) and serve as the standard packaging cell line for our Custom Adenovirus Services. These services are also available with our own RCA free (Replication Competent Adenovirus free) cell line BMAdE1™

Advantages

  • Superior sub-clone of 293A cells that adheres strongly to plastic dishes
  • Reliable post-crisis cell-line
  • Grow easily
  • Perform especially well in plaque assays and in transfection protocols
  • Ideal for the generation of helper independent recombinant adenovirus
  • Tested for the absence of mycoplasma

Ordering Information

Cat. # Description Size
AES0503 QBI-HEK 293A cells 1 mL (1 x 106 cells/mL)

QBI-HEK 293CymRCells

Description
This cell line stably expresses the repressor protein CymR under the control of the CMV5 promoter which makes them ideal for application with the Q-mate™ Inducible Expression System. In addition, this cell line contains the E1A and E1B Adenovirus type 5 genes that complement these genes deleted in a recombinant Adenovirus and is used for the generation of recombinant Adenovirus.

Advantages

  • Express stably the CymR repressor
  • Grow easily
  • Ideal for the generation of helper independent recombinant adenovirus
  • Contain the E1A and E1B Ad5 viral genes for complementation of E1 deleted recombinant adenovirusIdeal for the generation of helper independent recombinant adenovirus
  • Tested for the absence of mycoplasma

Ordering Information

Cat. # Description Size
AES2044 QBI-HEK293CymR cells 1 mL (1 x 106 cells/mL)

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