|
Amplification of a Recombinant Adenovirus
Description
Generation of a high-titer viral stock from a recombinant adenovirus.
Plaque purification prior to amplification is highly recommened
to insure the purity and stability of the recombinant adenovirus.
| Service No. |
Amplification Scale |
Infection of |
| ACS0200 |
500 mL |
5x108 cells |
| ACS0300 |
1 L |
1x109 cells |
| ACS0350 |
2 L |
2x109 cells |
| ACS0400 |
5 L |
5x109 cells |
| ACS0500 |
10 L |
1x1010 cells |
| ACS0600 |
20 L |
2x1010 cells |
| ACS0700 |
30 L |
3x1010 cells |
| ACS0800 |
40 L |
4x1010 cells |
| ACS0900 |
50 L |
5x1010 cells |
| ACS1000 |
60 L |
6x1010 cells |
| ACS1100 |
100 L |
1x1011 cells |
Time Required: 4-6 weeks
Includes:
- Amplification of a recombinant adenovirus in QBI-HEK 293A cells
at the desired volume.
- Collection of the adenovirus. Infected cells are harvested
and concentrated. Three freeze/thaw cycles are performed and cell
debris is removed by centrifugation.
- Delivery of supernatant containing recombinant adenovirus.
The final titer is variable and depends on the nature of the recombinant
adenovirus to be amplified. Typically, QBI-HEK 293A cells will produce
between 1,000 and 10,000 viral particles per cell. For an accurate
infectious titer, a TCID50 (ACS0070) or plaque assay
(ACS0060) can be performed on the final product.
For a complete list of MERLIN Custom Service catalog numbers, click
here.
For contact information, click
here.
|
