Construction of a Recombinant Adenovirus

Description
Recombinant adenoviruses are produced by in vivo homologous or transpositional recombination.

Service No. ACS0020X
Time Required: 2 months

Includes:

• Starting with a recombinant adenovirus transfer vector, E. coli or QBI-HEK293A cells will be transformed to yield recombinant adenoviral DNA.
• Generation of recombinant adenovirus by transfecting QBI-HEK 293A cells with recombinant viral DNA.
• Plaque purification and identification of the recombinant adenovirus by PCR or Western blot. If no amplicifcation is requested, eluted plaques are delivered to the client.

X = A: using Adeno-Quest™ system
X = B: using AdEasy™ system
X = C: using AdenoVator™ system
X = D: using Transpose-Ad™ system

For a table comparing different Transfer Vector options, click here.

For more information regarding Qbiogene's four Adenovirus Construction Technologies, click here.

For a complete list of MERLIN Custom Service catalog numbers, click here.

For contact information, click here.