Adenovirus Custom Services
   
Cloning ACS0010 Cloning gene of interest
Verification of Expression ACS0015 Transfection and screening by Western Blot
  ACS0016 PCR screening

Adenovirus  Construction*

X stands for:
AdEasy™
AdenoVator™
Transpose-Ad™
Adeno-Quest™
Technologies

 ACS0020X Construction of a recombinant adenovirus (clonal purity is ascertained)
  Includes:
ACS0033 Construction of recombinant adenoviral DNA
ACS0030 Plaque purification
ACS0100 Screening plaques by Western or PCR
  Construction of a recombinant adenovirus (clonal purity is assumed)
  Includes:
  Construction of recombinant adenoviral DNA
  Transfection of 293 cells
Plaque Purification
Prior to Amplification		 ACS0030 Plaque purification and screening of plaques by Western or PCR

Adenovirus Amplification

 Adenovirus production of high-titer stock
ACS0200 Scale:  500 mL culture
ACS0300 Scale:  1L culture
ACS0350 Scale:  2L culture
ACS0400 Scale:  5L culture
ACS0500 Scale:  10L culture
ACS0600 Scale:  20L culture above 20L, please inquire
Adenovirus Purification Adenovirus purification by CsCl banding.
ACS0040 For one liter of culture
ACS0045 For each subsequent liter of culture
Virus Titer Determination Determination of adenovirus titer by:
ACS0060 Plaque Assay (PFU/mL)
ACS0070 TCID50 (TCID50 units/mL)
ACS0075 OD260 (VP/mL)
Identity Test ACS0100 Western blot to confirm protein identity
Adenovirus Concentration ACS0050 Concentration of a purified recombinant adenovirus
Time Course & MOI Study ACS0080 Pilot project to determine optimal protein production
Protein Production Protein production
ACS2000 Scale:  500 mL culture
ACS3000 Scale:  1L culture
ACS3500 Scale:  2L culture
ACS4000 Scale:  5L culture
ACS5000 Scale:  10L culture
ACS6000 Scale:  20L culture above 20L, please inquire
Viral Quality ACS0060 Adenovirus Titration by Plaque Assay (PFU/mL)
ACS0070 Adenovirus Titration by TCID50 (TCID50/mL)
ACS0075 Adenovirus Titration by OD260 (VP/mL)
ACS0100 Identity Test: Western Analysis on Large Amplification Stock
ACS0090 Sterility Test
ACS0091 Mycoplasma Test
ACS0092 Endotoxin Test (by Gel Clot)
ACS0093 Endotoxin Test (Chromogenic)
ACS0094 RCA-PCR Assay

* Qbiogene can generate a recombinant adenovirus using other available technologies.






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