Adenoviral Vector Systems

Qbiogene Molecular Biology offers four different adenoviral expression systems for the production of recombinant adenovirus: Transpose-Ad™, AdenoVator™, AdEasy™, and AdenoQuest™ (see figure below). Two of these systems are based on homologous recombination in E. coli, one uses homologous recombination in human QBI-293A cells, and our most recent system takes advantage of site-specific transposition in E.coli.

The major differences between the four systems lie in the mode of recombination and the available transfer vectors. With each of the kits, several transfer vectors are available. Some transfer vectors come with a promoter and a poly A site, others benefit from a user-supplied expression cassette. Several of our transfer vectors are available with an MCS (multiple cloning site), whereas others have a single cloning site. In addition, the strength of the promoter, and therefore the level of protein expression, is an important consideration for the researchers when choosing the best kit for their research.

The plasmids that carry the Ad5 genome are all first generation, i.e. E1/E3. The expression cassette containing the researcher’s gene of interest is inserted into the deleted E1 region.

Each of our kits is available with the principal components and controls for the generation of 5 recombinant viruses and includes a comprehensive applications manual.

The following table shows the two major characteristics of each system: