Products and Services
   
Introduction
   
Biosafety Notice
   
Transpose-Ad™ Adenoviral Vector System
   
AdenoVator™ Adenoviral Vector System
   
AdEasy™ Adenoviral Vector System
   
Adeno-Quest™ Technology
   
Table comparing transfer vectors available at Qbiogene
   

 

Features of Qbiogene's transfer vectors

Name of
transfer vector		 Cloning
capacity		 Promoter Cloning
site		 Poly A
site		 Special
features
AdenoVator™
pAdenoVator-CMV5 6.1 kb CMV5 Pme I, Bam HI (1) Very strong CMV5 promoter for
constitutive expression
pAdenoVator-CMV5-IRES-GFP 4.6 kb CMV5 Bgl II (1) Very strong CMV5 promoter for
constitutive expression,
IRES-GFP coding region
pAdenoVator-CMV5-IRES-BFP 4.6 kb CMV5 Bgl II (1) Very strong CMV5 promoter for
constitutive expression,
IRES-BFP coding region
pAdenoVator-RSV 6.6 kb RSV MCS (3) RSV LTR enhancer and promoter sequences
pAdenoVator-SV40 6.8 kb SV40 Mlu I, Kpn I, Xho I (2) SV40 virus enhancer-promoter and origin of replication sequence
pAdenoVator-hCMV 6.4 kb CMV Sal I, Kpn I, Xho I (2) High and constitutive expression through CMV promoter
pAdenoVator-hCMV-Intron 6.2 kb CMV MCS (2) β-globin/IgG chimeric intron to achieve higher levels of transgene expression
pAdenoVator-Promoterless 7.2 kb User-supplied MCS (2) Large cloning capacity
Insert your own expression cassette
pAdenoVator-Promoterless-Intron 7.0 kb User-supplied MCS (2) Large cloning capacity
Insert your own expression cassette
β-globin/IgG chimeric intron to achieve higher levels of transgene
expression
pAdenoVator-CMV5- N(His)-1 6.2 kb CMV5 Nru I (2) Very strong CMV5 promoter for
constitutive expression
6x histidine tag for easy purification
of the fusion protein
pAdenoVator-CMV5-N(His)-2 6.2 kb CMV5 Nru I (2) Very strong CMV5 promoter for
constitutive expression
6x histidine tag for easy purification
of the fusion protein
pAdenoVator-CMV5-N(His)-3 6.2 kb CMV5 Nru I (2) Very strong CMV5 promoter for
constitutive expression
6x histidine tag for easy purification
of the fusion protein
pAdenoVator-CMV5-C(His)-1 6.2 kb CMV5 Nru I (2) Very strong CMV5 promoter for
constitutive expression
6x histidine tag for easy purification
of the fusion protein
pAdenoVator-CMV5-C(His)-2 6.2 kb CMV5 Nru I (2) Very strong CMV5 promoter for
constitutive expression
6x histidine tag for easy purification
of the fusion protein
pAdenoVator-CMV5-C(His)-3 6.2 kb CMV5 Nru I (2) Very strong CMV5 promoter for constitutive expression 6x histidine tag for easy purification of the fusion protein
pAdenoVator-CMV(CuO) 6.5 kb CMV(CuO) inducible promoter MCS (2) Strong inducible
CMV(CuO) promoter
pAdenoVator-CMV5(CuO) 6.0 kb CMV5(CuO) MCS (2) Very strong inducible
inducible promoter CMV5(CuO) promoter
pAdenoVator-CMV5(CuO)-IRES-GFP 4.5 kb CMV5(CuO) inducible promoter MCS (2) Very strong
inducible CMV5(CuO) promoter, IRES-GFP coding region
AdEasy™
pShuttle 7.5 kb User-supplied MCS - Largest cloning capacity
Insert your own expression cassette
pShuttle-CMV 6.6 kb CMV MCS (2) Strong CMV promoter
Transpose-Ad™  
pCR259 4.1 kb CMV MCS (2) β-globin/IgG chimeric intron for increased expression High and constitutive expression
through strong CMV promoter
pCR276 4.9 kb User-supplied MCS (2) β-globin/IgG chimeric intron for increased expression Insert your own expression cassette
Adeno-Quest™  
pQBI-AdBN 7.0 kb User-supplied MCS - Large cloning capacity
Insert your own expression cassette
pQBI-AdBM5-PAG 6.0 kb MLP Bam HI, Pme I (1) Modified MLP promoter
pQBI-AdCMV5 5.7 kb CMV5 Bam HI, Pme I (1) Very strong CMV5 promoter for
constitutive expression
pQBI-AdBM5-GFP 4.2 kb MLP Bgl II, Pme I (1) GFP coding region
pQBI-AdBM5-BFP 4.2 kb MLP Bgl II, Pme I (1) BFP coding region
pQBI-AdCMV5-IRES-GFP 4.2 kb CMV5 Bgl II (1) Very strong CMV5 promoter for
constitutive expression,
IRES-GFP coding region

Note:
(1): -globin Poly A signal
(2): SV40 Poly A signal
(3): BGH Poly A signal

CMV5: The CMV5 promoter was constructed by the insertion, downstream of the transcription start site of the human CMV IE (immediate early) promoter-enhancer, of the tripartite leader (tpl) and the splicing sites present in the human Adenovirus serotype 5 Major Late Promoter (MLP). The tpl binds translation-initiating proteins much more efficiently than most messages, resulting in a significant increase in protein expression levels compared to a standard CMV promoter.

IRES (Internal Ribosome Entry Segment): sequence supporting translation from the second cistron in a dicistronic message.






Home | About Adenovirus | Gene Therapy and Gene Delivery
Products and Services | Technical Support | References
Quality Control | Contact


.



Search Qbiogene/Products:
Advanced Search