|
Quality Control of the Adenoviral Vector Systems
Each lot of Adenoviral Vector Systems kits undergoes numerous and
stringent quality tests. Since the kits are made up of several components,
the following describes the test used to validate each component.
| Product/Test |
Plasmid DNA |
Viral DNA |
QBI-HEK 293A |
Competent Cells |
Buffers |
| Concentration by OD260 |
X |
X |
|
|
|
| DNA quality by gel analysis |
X |
X |
|
|
|
| Identity by restriction mapping |
X |
X |
|
|
|
| Functional by transient expression (plasmids expressing GFP
or BFP) |
X |
|
|
|
|
| Recombinant Adenovirus yield |
|
X |
|
|
|
| Mycoplasma |
X
(for certain plasmids) |
|
X |
|
|
| Endotoxin |
X
(for certain plasmids)
|
|
|
|
|
| Transfection Assay |
|
|
|
|
X |
| Viability |
|
|
X |
|
|
| Sterility |
X |
X |
X |
|
X |
| Cell concentration |
|
|
X |
|
|
| Infectivity |
|
|
X |
|
|
| Verification of competency by transformation
assay |
|
|
|
X |
|
| Volume verification |
X |
X |
X |
X |
X |
|
