New Products

 New Total Chromosome Paint Probes
 Arm Specific Probes
 HER-2/neu Probe
 New Improved Microdeletion probes
 Iso17 (MPO/p53) Probe
 Inv16 split Probe
 MLL (11q23) split probe

The new probes are compatible with the current version of paints, and combination of old and new probes will be possible. Please use our Universal FISH protocol for all Qbiogene probes. Due to this new development Qbiogene has now got total chromsome paint probes Blue in a 5-test format for the following chromosomes .

Human Chromosome Arm Specific Probes
As a result of our Band Specific Custom Service, we are now introducing the full range of Arm Specific Paints (ASP's) available in two colours (Red & Green).  These Arm Specific Paints allow a more refined research tool to be used in the investigation of complex chromosomal re-arrangements and marker chromosome identification.  The ASP's are complementary to our regular Total Chromosome Paints and general probe offer.
 
The Arm Specific probes are obtained from microdissected DNA material and are directly labeled with a fluorochrome using PCR techniques.  They are generated and quality controlled to provide accurate chromosome arm specific coverage and brilliant high fluorescence signals when attendance to protocol is observed.
 
The specific advantages of this new probe technology being:
 
* Simple to use
* Rapid methodology
* Bright and accurate signals
* High Fluorescence Intensity
* Excellent Signal Specificity
 
These direct labeled probes have been tested and qualified on cultured cells and are optimized for hybridization on metaphase spreads from cytogenetic preparations. They can be used for identifying whole human chromosomes, translocation events, chromosome re-arrangements and determining the origin of marker chromosomes.  Mutagenesis analysis, radiation and sensitivity testing, identification of human chromosomes in hybrid cells can also be investigated using these probes.

Technical Information
The Qbiogene Arm Specific Paints (ASP's) are available with a Green Fluorochrome label (ex. 495/em. 525) and a Red Fluorochrome label (ex.  565/em. 590). Please note that only the q-arm is available for the acrocentric chromosomes (Nr. 13, 14, 15, 21, 22 and Y). Heterochromatic areas (e.g. 1qh, 9qh and Yq) will not be covered by this type of probe. Due to diffuse signal these Arm Specific Probes are not recommended for Interphase cell analysis. The Arm Specific probes are provided in their own Hybridization buffer and are ready to use following the suggested protocol in each package insert. The volume of each vial is 100 µL for the recommended 10 test analysis.

Ordering Information

Figure 4.
1p/1q hybridization in red and green

Alterations of the HER-2/neu proto-oncogene have been implicated in the carcinogenesis and prognosis of breast cancer. The HER-2/neu (also called ERBB2) gene encodes a 185 kDa transmembrane cell surface glycoprotein and is a member of the tyrosine kinase family with a high degree of homology to the epidermal growth factor (EGF-R). Amplification of HER-2/neu has also been reported in prostate carcinoma, uterus endometrial cancer and primary gastric cancer.

The new developed Qbiogene probe for HER-2/neu, labeled with Rhodamine (red), uses the Universal Linkage System (ULS^®) and is optimized to detect amplification of the HER-2/neu gene region. The included Chromosome 17 Alpha-Satellite probe, labeled with FITC (green), serves as internal control and simultaneously defines the ploidy status of Chromosome 17.

Localization: Locus: 17q11.2 – q12, Gene: HER-2/neu (ERBB2), Probe Size : 450 kb

Ordering Information

Related Product
PONCHER2 Probe and reagents for 50 HER-2/neu assays

Description of the HER-2/neu probe in PDF format - Click here

Is17qMet		Iso17qInt

A less frequent event in AML, but a common secondary change in CML is the isochromosome for the long arm of chromosome 17. It results in the loss of the p-arm of chromosome 17 distal to the Smith-Magenis gene region at 17p11 and a duplication of the remaining 17q which often results in dicentric chromosome. The Qbiogene Iso 17q probe covers the entire p53 region at 17p12 labeled in green and the MPO gene region in red. In normal cases two green and two red signals are observed, in Iso17q patients the signal pattern is one green and three red signals.

Probe size: 200 kb (p53) green, and195 kb (MPO) red
Locus: 17p12 and 17q23

Inv16

The Inversion (16)(p13q22) chromosomal rearrangement results in the fusion of CBFB and MYH11 genes and is one of the most frequent chromosomal abnormalities observed in AML. The Qbiogene Inv(16) probe contains differently labeled regions adjacent to the CBFB gene region. In normal cells two red-green (or yellow) fusions signals are observed, in case of an inversion or translocation one signal will split into separate green and red signals. As a deletion within MYH at 16p13 often accompany the 16p13/16q22 rearrangement (in 20% of cases), the CBFB locus has been choosen.

Probe size: 200 kb, red and 205 kb, green
Locus: 16q22

MLLSplit

Reciprocal translocations involving the MLL gene on chromosome band 11q23 have been observed in both acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL). In AML, identification of MLL breakpoints is an important prognostic factor. The Qbiogene MLL (11q23) split probes contains differently labeled regions adjacent to the MLL beakpoint region. In normal cells two red-green (or
yellow) fusion signals are observed, in case of a translocation on red-green (or yellow) fusion signals and one separate green and red signal will be visible.

Probe size: 250 kb, red and 300 kb, green
Locus: 11q23