|
|
|
Telomerase
Probes (hTERT;hTERC)
A set of
probes for 3q26 (hTERC) and 5p15 (hTERT) is now available from Qbiogene,
manufactured using the Universal Linkage System (ULS )
These direct
labeled probes have been tested and qualified on cultured cells, blood
smears and paraffin-embedded tissue
sections.
3q26 hTERC
- Human RNA component for Telomerase
A common amplification target of encompassing chromosome region 3q25 to
q27 has been identified by comparative genomic hybridization analyses
in tumors of the cervix, ovary, endometrium, lung, and head and neck.
The region of highest amplification included the gene for the RNA component
of telomerase (hTERC), supporting it as a potential target. Although the
importance of low-level amplification is unknown, the consistent and reproducible
nature of this event in a variety of carcinomas suggests that 3q26.2 harbors
an oncogene whose low-level amplification has a significant influence
on tumor biology. (Cancer Genet Cytogenet 2000 Feb;117(1):9-18)
5p15 hTERT
– Catalytic subunit of Human Telomerase
The gene for the reverse transcriptase component of telomerase (hTERT)
has been mapped by fluorescence in situ hybridization (FISH) to chromosome
5p15.33. Increase in copy number of the hTERT gene region do occur, and
may well be one route to upregulating telomerase levels in tumour cells.
5p15 gains and amplifications have been documented for various tumour
types, including non-small cell lung carcinoma, squamous cell carcinoma
of head and neck, and uterine cervix cancer, making hTERT a potential
target. (Oncogene 1998 Mar 12;16(10):1345-50)
The 5p15 hTERT probe is also available in combination with a 5q31(D5S89)
specific probe as dual-color assay. The 5q31 probe can serve as internal
control to verify deletions or amplification of 5p15.
Ordering
Information
| Cat
No. |
Probe
Description |
Label |
Pack
Size |
| PONC0530 |
5p15.33
(hTERT) / 5q31(D5S89) probe cocktail |
Dual-color |
100
µl |
| PONC0515 |
5p15
(hTERT) specific DNA Probe |
Green |
100
µl |
| PONC0320 |
3q26
(hTERC) / Alphasatellite 3 probe cocktail |
Dual-color |
100
µl |
| PONC0321 |
3q26
(hTERC) |
Red |
100
µl |
*
The Universal Linkage System (ULS®) technology is covered by an international
patent family for the linkage of any label to DNA, RNA, nucleotides or
proteins, owned by KREATECH Biotechnology BV, The Netherlands, including,
but not restricted to, the following: EP 0539466; US 5,580,990; US 5,714,327;
WO 92/01699; WO 96/35696; WO 98/15564.
|
