siRNA transfection reagent
permits high level suppression of endogenous gene expression
jetSI is a new transfection reagent optimized for efficient delivery
of siRNA. This reagent has been shown to provide 80% knock-out of target
gene expression in a variety of cell lines. Below are the results obtained
when jetSI was used to transfect siRNA directed against endogenous
lamin A/C gene in HeLa cells.
Immunofluorescence staining of Hela cells transfected with lamin A/C
siRNA duplex after 72 h, using jetSI, visualized by fluorescence
(A) control (no transfection);
(B) transfection of 100nM of lamin A/C siRNA;
(C) transfection of 100 nM of mismatch (Mm) lamin A/C siRNA .