Easy lysis of yeast cells to release recombinant proteins
The FastProtein Red Matrix, also known as Lysing Matrix C, is used with the FastPrep® Instrument to reproducibly lyse yeast cells within 40 seconds. A brief protocol is provided to allow for the maximum flexibility in lysis and purification methods. If no special induction buffer is required, the user can lyse yeast cells in the provided Yeast Breaking Buffer. Rapid release of recombinant protein saves valuable time during optimization of expression. Information from up to 12 different induction variables can be quickly obtained after one 40 second FastPrep run followed by electrophoresis. Once your protein of interest is expressed and identified, the active proteins released by the unique motion of the FastPrep Instrument can be recovered from the lysate by a wide range of protein-specific techniques.