FastRNA® Pro Green Kit

Reference Library
  Protocol
  Profile

Isolation of total RNA from plant and animal samples

Advantages

  • Rapid and reproducible sample lysis in under 40 seconds with the FastPrep® Instrument
  • Difficult samples such as roots, stems, seeds, and cartilage lysed with ease
  • RNA safely and consistently isolated with single-reagent RNApro™ Solution
  • Lyse, isolate and precipitate RNA in less than 90 minutes

The FastRNA Pro Green Kit is designed to efficiently isolate total RNA from any type of plant and animal tissue or cultured cells. Using the FastPrep Instrument, between 50-500 mg of tissue can be homogenized by Lysing Matrix D in impact-resistant 2 ml tubes. Total RNA is released into the proprietary, protective RNApro™ Solution. Following extraction with chloroform and precipitation, total RNA is suitable for RT-PCR analysis and other downstream applications.

Rat total RNA extracted with the
FastRNA Pro Green Kit.

Approximately 2% of the total RNA isolated from 100 mg frozen tissue was loaded on to a 1.2% denaturing agarose gel (1X MOPS). Lane 1: tail; Lane 2: kidney; Lane 3: liver; Lane 4: ear; Lane 5: brain; Lane 6: 0.24-9.5kb RNA Ladder.
Plant total RNA extracted with the
FastRNA Pro Green Kit.

Approximately 2% of the total RNA isolated from 100 mg tissue was loaded on to a 1.2% denaturing agarose gel (1X MOPS). Lane 1: wheat seed; Lane 2: tomato leaf; Lane 3: red soybean seed; Lane 4: oat seed; Lane 5: iceplant leaf; Lane 6: barley seed; Lane 7: 0.24-9.5kb RNA Ladder.
Kit Components: Lysing Matrix D, RNApro™ Solution, DEPC-treated H2O

Cat # Description Size
6045-050 FastRNA® Pro Green Kit 50 preps

 

FastPrep® Products

The FastPrep® Instrument
FastDNA® Kit
FastDNA® SPIN Kit
FastDNA® SPIN Kit for Soil

FastRNA® Pro Kits
FastRNA® Pro Soil-Direct Kit
FastRNA® Pro Soil-Indirect Kit
FastProtein™ Matrices

 

   




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