Isolation of total RNA directly from soil
Advantages
- Rapid
and reproducible sample lysis in under 40 seconds with the FastPrep® Instrument
- Easily
lyse difficult gram positive cells, plant material, and organic debris
directly from soil
- Lysis
and purification solutions protect RNA during processing
- Humic
acids levels reduced to allow uninhibited RT-PCR
- From
lysis to total RNA in less than 2 hours
The
FastRNA Pro
Soil-Direct Kit is designed to efficiently isolate
total RNA from organic material found in soil samples.
Using the FastPrep Instrument,
up to 0.5 g of material is homogenized by Lysing
Matrix E in impact-resistant 2 ml tubes. Total RNA
is released into a protective solution, extracted,
precipitated, and cleaned with the proprietary RNAMATRIX® and
a Quick-Clean Spin Filter. Total RNA prepared with
this kit is suitable for RT-PCR analysis and other
downstream applications.
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Kit
Components: RNApro Soil
Lysis Solution, Lysing Matrix E, Phenol:Chloroform
(1:1), Inhibitor Removal Solution, DEPC-treated H2O, RNAMATRIX® ,
Binding Solution, RNAMATRIX® Slurry,
RNAMATRIX® Wash
Solution Concentrate, Quick-Clean Spin Filters
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