Isolation of total RNA from soil supernatants

Advantages

• Rapid and reproducible sample lysis in under 40 seconds with the FastPrep^® Instrument
• Easily lyse difficult gram positive cells, plants, and other cellular material
• Lysis and purification solutions protect RNA during processing
• Humic acids levels enormously reduced to allow uninhibited RT-PCR
• From lysis to total RNA in less than 2 hours

The FastRNA Pro Soil-Indirect Kit is designed to efficiently isolate total RNA from organic material found in soil sample supernatants. Cellular material is washed away from soil particles, centrifuged, and homogenized by the FastPrep Instrument in impact-resistant 2 ml tubes of Lysing Matrix E. Total RNA is released into a protective solution, extracted, precipitated, and cleaned with the proprietary RNAMATRIX^® and a Quick-Clean Spin Filter. Total RNA prepared with this kit is suitable for RT-PCR analysis and other downstream applications.