Recombinant Adenovirus Production

AdEasy™ Adenoviral Vector System
The AdEasy™ Adenoviral Vector System, based on homologous recombination in E. coli cells (BJ5183), is 100% compatible with the AdenoVator™ system. The transfer vectors of the AdEasy™ system can be used with the AdenoVator™ technology and vice versa.

Advantages

  • No plaque purification required
  • Gene transfer accomplished using the highly efficient homologous recombination apparatus of E. coli
  • Vector selection allows you to subclone your own expression cassette, including your choice of promoter and polyA signal
  • 100% compatible with the AdenoVator™ Adenoviral Vector System

Ordering Information
The Basic Kit contains the following components:
BJ5183 EC (electrocompetent)
DH5α EC (electrocompetent)
pAdEasy-1
QBI-Infect
Transfection reagents (2M CaCl2, 0.1X TE and 2X HBS)

Cat#
Basic
Kit
Reagents provided transfer vectors
QBI-HEK 293A cells
AES1000    Y                  none                            Y
AES1000A  Y      pShuttle and pShuttle-CMV          Y
AES1001    Y                  none
AES1001A  Y      pShuttle and pShuttle-CMV

Products sold separately

Cat# Description Volume
AES1010    pAdEasy-1, the 33.4 kb plasmid
                containing the ∆E1/E3 Adenovirus
                serotype 5 genome                             2.5 µg
AES1020    pShuttle transfer vector                        25 µg
AES1021    pShuttle-CMV transfer vector                 25 µg
AES1009    BJ5183 EC DH5α EC                    5 tubes each
AES1005K  BJ5183 EC                                       5 X 80 µL
AES1007K  DH5α EC                                          5 X 40 µL
AES0503    QBI-HEK 293A cells                              1 mL*
AES1015    Refill kit: pAdEasy-1 BJ5183 EC             Kit
AES1016    Refill kit: pAdEasy-1 BJ5183 EC
                 DH5α EC                                            Kit

 

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