Recombinant Adenovirus Production

Adeno-Quest™ Adenoviral Vector System
This Adenoviral vector system is based on in vivo homologous recombination in human QBI-HEK 293A cells. The principle of the system is simple: the gene of interest, or the complete expression cassette (including promoter, gene of interest and polyA signal), is sub-cloned into a transfer vector, which is
introduced together with linear viral DNA into human QBIHEK 293A cells. The subsequent in vivo homologous recombination yields recombinant viral particles which are transduced into QBI-HEK 293A cells for amplification.

Advantages

  • Investigators, who feel more comfortable with virology techniques than with molecular biology methods can use this system
  • Proven method that has given full satisfaction to many researchers over a number of years

Ordering Information
QBI-Viral DNA
QBI-Transf
QBI-Infect
QBI-HEK 293A cells
Transfection reagents (2M CaCl2, 0.1X TE and 2X HBS)

Cat#
Reagents provided
Transefer vector
QBI-HEK 293A cells
AES9500           Y                 none                    Y
AES050D           Y         pQBI-AdBM5-PAG          Y
AES050E           Y               pQBI-AdBN              Y
AES050G           Y         pQBI-AdBM5-GFP          Y
AES050H           Y         pQBI-AdBM5-BFP          Y
AES050L            Y          pQBI-AdCMV5             Y
AES050M           Y   pQBI-AdCMV5-IRES-GFP      Y

Products sold separately

Cat# Description Volume
AES0501    QBI-viral DNA                               25 µg
AES0503    QBI-HEK 293A cells                        1 mL*
AES0521    pQBI-AdBN                                   25 µg
AES0523    pQBI-AdBM5-GFP                          25 µg
AES0524    pQBI-AdBM5-BFP                          25 µg
AES0527    pQBI-AdCMV5-IRES-GFP                25 µg
AES0535    pQBI-AdCMV5                              25 µg
AES0537    pQBI-AdBM5-PAG                          25 µg
 

 

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