Adenoviral Vector System
Qbiogene introduces the new AdenoVatorAdenoviral Vector System for maximal protein expression and fluorescence detection of transduced mammalian cells. Using the AdenoVatorAdenoviral Vector System, 5-10 fold higher protein expression (up to 30% of total cellular protein) can be achieved in the target cell of interest compared to other commercially available CMV-based systems. Additionally, the AdenoVatorsystem offers a choice of co-expression shuttle vectors to analyze the expression of your gene of interest along with the fluorescent tags SuperGloGFP or SuperGloBFP.
Increased Protein Expression
AdenoVatorshuttle vectors use the powerful chimeric CMV5 promoter to drive expression of your gene of interest. The CMV5 promoter consists of the CMV promoter and enhancer with the adenovirus major late enhancer and splicing sequence and the major late protein tripartite leader sequence resulting in higher expression than the conventional CMV promoter in an equally broad range of cell types. Using CMV5 as a promoter, an increased protein yield is obtained with each adenovirus infection. Yields of up to 30% of Total Cellular Protein (TCP) in both complementing (293) and non-complementing cell lines are easily obtained, providing enhanced protein yields for purification purposes. In whole animal experiments, increased protein expression translates into a potentially-reduced immune response since less adenovirus is required for infection.
Co-expression with GFP or BFP
The pAdenoVator-CMV5-IRES-GFP and pAdenoVator-CMV5-IRES-BFP transfer vectors are designed to rapidly identify and quantify recombinant adenovirus transduced cells expressing your gene of interest, in vivo or in vitro. These vectors utilize the powerful CMV5 promoter to drive expression of a dicistronic cassette comprised of your gene of interest and either SuperGloGFP or SuperGloBFP linked to an Internal Ribosome Entry Segment (IRES). Functional characterization of genes of interest in specific target cells is greatly simplified since recombinant adenovirus expressing sgGFPor sgBFPcan be readily identified by conventional methods such as fluorescence microscopy or flow cytometry.
Easy-to-use E. coli-based System
As with our existing AdEasyVector System, the construction of a recombinant adenoviral vector is a two-step process in which the desired expression cassette is first subcloned into a shuttle vector, and subsequently transferred into the adeno-viral genome by means of homologous recombination in E.coli. This method saves weeks of time and requires simpler selection of recombinant adenovirus vectors compared to traditional methods. The AdenoVatorAdenoviral Vector System is fully compatible with our AdEasySystem.
He, T. C., S. Zhou, et al. (1998). "A simplified system for generating recombinant adenoviruses." Proc Natl Acad Sci U S A 95(5): 2509-14.
Mosser, D. D., A. W. Caron, et al. (1997). "Use of a dicistronic expression cassette encoding the green fluorescent protein for the screening and selection of cells expressing inducible gene products." Biotechniques 22(1): 150-161.