AdenoVator™ Adenoviral Vector System

Qbiogene introduces the new AdenoVator™Adenoviral Vector System for maximal protein expression and fluorescence detection of transduced mammalian cells. Using the AdenoVator™Adenoviral Vector System, 5-10 fold higher protein expression (up to 30% of total cellular protein) can be achieved in the target cell of interest compared to other commercially available CMV-based systems. Additionally, the AdenoVator™system offers a choice of co-expression shuttle vectors to analyze the expression of your gene of interest along with the fluorescent tags SuperGlo™GFP or SuperGlo™BFP.

Increased Protein Expression

AdenoVator™shuttle vectors use the powerful chimeric CMV5 promoter to drive expression of your gene of interest. The CMV5 promoter consists of the CMV promoter and enhancer with the adenovirus major late enhancer and splicing sequence and the major late protein tripartite leader sequence resulting in higher expression than the conventional CMV promoter in an equally broad range of cell types. Using CMV5 as a promoter, an increased protein yield is obtained with each adenovirus infection. Yields of up to 30% of Total Cellular Protein (TCP) in both complementing (293) and non-complementing cell lines are easily obtained, providing enhanced protein yields for purification purposes. In whole animal experiments, increased protein expression translates into a potentially-reduced immune response since less adenovirus is required for infection.

Co-expression with GFP or BFP

The pAdenoVator™-CMV5-IRES-GFP and pAdenoVator™-CMV5-IRES-BFP transfer vectors are designed to rapidly identify and quantify recombinant adenovirus transduced cells expressing your gene of interest, in vivo or in vitro. These vectors utilize the powerful CMV5 promoter to drive expression of a dicistronic cassette comprised of your gene of interest and either SuperGlo™GFP or SuperGlo™BFP linked to an Internal Ribosome Entry Segment (IRES). Functional characterization of genes of interest in specific target cells is greatly simplified since recombinant adenovirus expressing sgGFP™or sgBFP™can be readily identified by conventional methods such as fluorescence microscopy or flow cytometry.

Easy-to-use E. coli-based System

As with our existing AdEasy™Vector System, the construction of a recombinant adenoviral vector is a two-step process in which the desired expression cassette is first subcloned into a shuttle vector, and subsequently transferred into the adeno-viral genome by means of homologous recombination in E.coli. This method saves weeks of time and requires simpler selection of recombinant adenovirus vectors compared to traditional methods. The AdenoVator™Adenoviral Vector System is fully compatible with our AdEasy™System.

Cat No.	Product
AES2000A	AdenoVatorTM Kit with pAdenoVator-CMV5 transfer vector and HEK 293 cells
AES2000B	AdenoVatorTM Kit with pAdenoVator-CMV5-IRES-GFP transfer vector and HEK 293 cells
AES2000C	AdenoVatorTM Kit with pAdenoVator-CMV5-IRES-BFP transfer vector and HEK 293 cells
AES2001A	AdenoVatorTM Kit with pAdenoVator-CMV5 transfer vector
AES2001B	AdenoVatorTM Kit with pAdenoVator-CMV5-IRES-GFP transfer vector
AES2001C	AdenoVatorTM Kit with pAdenoVator-CMV5-IRES-BFP transfer vector
AES2010	pAdenoVator Vector
AES2015	pAdenoVator & BJ5183 cells
AES2016	pAdenoVator & BJ5183 cells & DH5
AES2022	pAdenoVator-CMV5 Vector
AES2023	pAdenoVator-CMV5-IRES-GFP Vector
AES2024	pAdenoVator-CMV5-IRES-BFP Vector

He, T. C., S. Zhou, et al. (1998). "A simplified system for generating recombinant adenoviruses." Proc Natl Acad Sci U S A 95(5): 2509-14.

Mosser, D. D., A. W. Caron, et al. (1997). "Use of a dicistronic expression cassette encoding the green fluorescent protein for the screening and selection of cells expressing inducible gene products." Biotechniques 22(1): 150-161.