AdenoVator™ Adenoviral Vector System
The AdenoVator™ Adenoviral Vector System is based on homologous recombination in E. coli cells (BJ5183) between a transfer vector and a plasmid containing the E1/E3 deleted Ad5 (Adenovirus 5) genome (pAdenoVator ∆E1/E3). The gene of interest is cloned into the transfer vector which is subsequently co-transformed with the Ad5-backbone containing plasmid into BJ5183 cells where the homologous recombination will take place. The next step consists in linearizing the recombinant DNA and transferring it into QBIHEK 293A cells to ensure the generation and amplification of viral particles.

Advantages

• Gene transfer accomplished using the highly efficient homologous recombination apparatus of E. coli cells
• No plaque purification required
• Largest choice of transfer vectors
• Can be used in combination with the Q-mate™ inducible expression system
• Highest level of protein expression available by using the CMV5 promoter (up to 35% of total cellular protein)
• Rapid identification and quantification of co-expressed genes using GFP or BFP
• 100% compatible with the AdEasy™ adenoviral vector system

† contain QBI-HEK 293CymR cells instead of QBI-HEK 293A cells

*1 mL = 1 x 10⁶ cells

Q-mate™ Controllable Transfer Vectors
Qbiogene now also offers several transfer vectors that have been designed for controllable gene expression using the Q-mate™ Inducible Expression System.

These vectors contain the operating sequences for the CymR repressor (CuO) linked to either the CMV or the CMV5 promoter, which is 5 to 10 times stronger than the CMV promoter. pAdenoVator-CMV5(CuO)-IRES-GFP carries the IRES sequence and the GFP coding region which allow the co-expression of the protein of interest and the GFP.

For more information on the Q-mate™ system, please click here.

CMV5: The CMV5 promoter was constructed by the insertion, downstream of the transcription start site of the human CMV IE (immediate early) promoter-enhancer, of the tripartite leader (tpl) and the splicing sites present in the human Adenovirus serotype 5 Major Late Promoter (MLP). The tpl binds translation-initiating proteins much more efficiently than most messages, resulting in a significant increase in protein expression levels compared to a standard CMV promoter.

IRES (Internal Ribosome Entry Segment): sequence supporting translation from the second cistron in a dicistronic message.

For licensing information, please refer to the end of this section.

AdenoVator™ system to generate recombinant Adenovirus expressing the transgene under the Q-mate™ Inducible Expression System.