Q-mate™ Controllable Transfer Vectors
Qbiogene now also offers several transfer vectors that have been designed for controllable gene expression using the Q-mate™ Inducible Expression System.
These vectors contain the operating sequences for the CymR repressor (CuO) linked to either the CMV or the CMV5 promoter, which is 5 to 10 times stronger than the CMV promoter. pAdenoVator-CMV5(CuO)-IRES-GFP carries the IRES sequence and the GFP coding region which allow the co-expression of the protein of interest and the GFP.
For more information on the Q-mate™ system, please click here.
CMV5: The CMV5 promoter was constructed by the insertion, downstream of the transcription start site of the human CMV IE (immediate early) promoter-enhancer, of the tripartite leader (tpl) and the splicing sites present in the human Adenovirus serotype 5 Major Late Promoter (MLP). The tpl binds translation-initiating proteins much more efficiently than most messages, resulting in a significant increase in protein expression levels compared to a standard CMV promoter.
IRES (Internal Ribosome Entry Segment): sequence supporting translation from the second cistron in a dicistronic message.
For licensing information, please refer to the end of this section.