| Q-mate™ Controllable Transfer Vectors
Qbiogene now also offers several transfer vectors that have been
designed for controllable gene expression using the Q-mate™
Inducible Expression System.
These vectors contain the operating sequences for the CymR
repressor (CuO) linked to either the CMV or the CMV5 promoter,
which is 5 to 10 times stronger than the CMV promoter.
pAdenoVator-CMV5(CuO)-IRES-GFP carries the IRES
sequence and the GFP coding region which allow the co-expression
of the protein of interest and the GFP.
For more information on the Q-mate™ system, please click here.
CMV5: The CMV5 promoter was constructed by the insertion,
downstream of the transcription start site of the human CMV
IE (immediate early) promoter-enhancer, of the tripartite
leader (tpl) and the splicing sites present in the human
Adenovirus serotype 5 Major Late Promoter (MLP). The tpl
binds translation-initiating proteins much more efficiently
than most messages, resulting in a significant increase in protein expression levels compared to a standard CMV promoter.
IRES (Internal Ribosome Entry Segment): sequence supporting
translation from the second cistron in a dicistronic message.
For licensing information, please refer to the end of this section.
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