Started: Reporter AdenoExpress
Prior to starting a recombinant adenovirus production we recommend you test your specific in vitro or in vivo model with either GFP or LacZ Reporter AdenoExpress. These are ready-made, high titer recombinant adenoviruses that serve to optimize transduction parameters such as determining the optimal MOI (Multiplicity of Infection) needed to infect a specific cell type as well as the amount of virus that will be required to perform the entire research project (the level of large-scale production).
Reporter AdenoExpress can also be used as a control in experimental protocols. GFP offers an advantage over LacZ in that it can be used in real time assays in living cells, whereas LacZ can be detected by a simple colorimetric assay.
Transduction efficiencies vary with the number of Adenovirus cell surface receptors (the CAR, or "Coxsackie virus and Adenovirus Receptor", and the vß3 or vß5 integrins), which differs greatly in number from one cell line to another. Preliminary experiments and evaluation studies using AdenoExpress represent the most reliable and convenient methods of getting started with adenoviral vector technologies.
For each Reporter AdenoExpress two titers are available: