TRAPeze® Telomerase Detection Kit
The Qbiogene TRAPeze® Kit is based on the TRAP assay to
detect Telomerase activity in cells. In the assay, active
Telomerase adds telomeric repeats to an artificial template (TS
= Telomerase Substrate). The extended products are amplified;
generating a ladder of products with 6-base increments starting
at 50 bp. Detection can be made by gel electrophoresis
using Ethidium Bromide, Syber Green, Radiography or Silver
staining methods or with the specific TELactive Elisa kit.
Each reaction mixture contains a control primer and a telomerase
substrate control for amplification of a 36 bp internal
standard. Incorporation of this internal positive control makes
it possible to quantitate telomerase and, in addition, to identify
false negative samples that contain Taq polymerase inhibitors.
The kit provides sufficient reagents to perform 112 assays. With these reagents, at least 40 samples with appropriate positive and negative controls can be analysed.
Kits Components: CHAPS Lysis buffer, 10 X reaction buffer, 50 X dNTP Mix,
TS Primer, Primer Mix, PCR-Grade water, TSR8 (control template), control cell
pellet (106 cells)
|S7700-KIT TRAPeze® Telomerase
Detection Kit 112 assays
S7705 TRAPeze® 1xCHAPS
Lysis Buffer 8.2 mL