TRAPeze® ELISA Telomerase Detection Kit
||The TRAPeze® ELISA Kit uses the TRAP extension and
amplification methodology, as in the TRAPeze® kit. The
TRAP extension/amplification reaction is performed
with biotinylated primer and DNP-labelled dCTP. Thus, the TRAP products are tagged with biotin and DNP
residues. The labeled products are immobilized onto
streptavidin-coated microtiter plates via biotin-streptavidin interaction, and then detected byanti-DNPantibody conjugated to horseradish peroxidase (HRP). The amount of
TRAP products is determined by means of the HRP activity using substrates 3,3’,5,5’ - tetramethylbenzidine (TMB) and subsequent colour development. While the method is non-quantitative, telomerase activity in extracts made from 30- 1000 telomerase positive cells can readily be measured. The procedures of TRAPeze® ELISA kit are optimized for extremely sensitive and specific detection of telomerase activity in clinical research samples. Telomerase activity from as few as 30 telomerase positive cells among 3000 negative cells can be detected with the TRAPeze® ELISA kit.
Many clinical research samples contain Taq polymerase
inhibitor(s), which may cause potential false-negative results. Dilution of the sample minimizes the effect of inhibitors. However, as an aid to data interpretation, direct visualization of the TRAP ladder is easily accomplished by polyacrylamide gel electrophoresis and staining with SYBR® Green or ethidium bromide. The presence or absence of the signature telomerase
ladder confirms the ELISA results.
This ELISA kit provides sufficient reagents to perform 96
reactions and ELISA detections. With these reagents, 28 samples
with appropriate positive and negative controls can be
Kit Components: CHAPS Lysis buffer, 5X TRAP reaction mix, PCR grade water,
TSR8 template, Positive control cell pellet (106 cells), Anti-DNP Antibody, HRP
conjugated, Blocking/detection buffer, 10X washing buffer, TMB solution
Stop solution, Streptavidin-coated Microtiter Plate.
|S7750-KIT TRAPeze® ELISA
Telomerase Detection Kit 96 assays