ApopTag® Direct Fluorescein in situ
This kit detects DNA fragmentation by the catalytic addition
of fluorescein-nucleotide residues to the DNA ends. The
incorporated nuceleotides form a random heteropolymer in a
ratio that has been optimized. Using a nucleotide directly
labeled with a fluorophore allows for fewer manipulations,
giving good cell recovery and approximately equal mean signal
strength compared to affinity labeling methods. Qualified
for use in research samples such as cell suspensions, formalin-fixed paraffin-embedded tissues, cryostat sections and
Kit Components: Equilibration buffer, reaction buffer, TdT enzyme, stop/wash
buffer, blocking solution, anti-digoxigenin-fluorescein.
|S7160-KIT ApopTag® Direct Kit 40 tests
ApopTag® Control Slides for in situ
- Positive control for apoptosis
- For microscopy applications
- Teaching aid
This product is a positive control for use with the ApopTag® in
situ Detection Kits. These reference slides are also useful as
teaching aids, and for procedural troubleshooting.
The rat mammary gland undergoes programmed cell death
processes during the tissue regression that follows lactation.
Apoptotic bodies, which can be seen by routine histological
staining, can be specifically stained by techniques that localize
DNA strand breaks in situ. The specificity of staining for
strand breaks can be correlated with the morphological
changes that occur during apoptosis.
|S7115 ApopTag® Control Slides 5/pack