ApopTag® Fluorescent in situ Detection Kits
TUNEL kit for use with fluorescent-light microscopes
ApopTag® Fluorescent in situ Detection Kits detect apoptotic cells by direct fluorescent detection of digoxigenin-labelled genomic DNA. ApopTag® Fluorescein Kit and ApopTag® Red Kits employ Fluorescein and sulforhodamine tagged antidigoxigenin antibodies, respectively. The ApopTag® Fluorescein Plus kit includes positive control slides to aid identification of apoptotic cells.
Principles of the procedure: Apoptotic cells are detected in situ by specific end labeling and detection of the DNA fragments produced by the apoptotic process. Residues of digoxigeninnucleotide
are catalytically added to the DNA by terminal
deoxyribonucleotidyl transferase (TdT). The anti-digoxigenin
antibody fragment carries a fluorescein molecule to the reaction
site. The localized signal generated allows for the sensitive
and specific staining of the very high concentration of 3’-OH
ends that are localized in apoptotic bodies. Kit Components: Equilibration Buffer, Reaction Buffer, TdT Enzyme, Stop/Wash
Buffer, Blocking Solution, Anti-Digoxigenin-Peroxidase 
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S7110-KIT ApopTag® Fluorescein 40 assays
S7111-KIT ApopTag® Plus Fluorescein 40 assays
S7165-KIT Apoptag® Red 40 assays
S7115 Control Slides 5 slides
S7106 Equilibration Buffer 1 vial (15 ml)
S7105 Reaction Buffer 1 vial (1 ml)
S7107 TdT Enzyme 1 vial (300 µl)
S7108 Stop/Wash Buffer 1 vial (20 ml) |
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