Methylation Specific PCR

CpGenome™ DNA Modification Kit
The CpGenome™ Modification Kit contains reagents required to perform a bisulfite modification on a DNA sample. In the reaction all unmethylated cytosines are deaminated and converted to uracils, while 5-CH3-Cytosines remain unaltered. The sequence of the treated DNA will differ if the DNA is originally
methylated versus unmethylated. Specific primers can be designed to amplify each of the sequences based upon these chemically-induced differences or the modified DNA can be sequenced.

Note: Sufficient DNA can be recovered to perform MSP on as little as 0.002 µg of DNA.

Technical Information
Pack Size: Sufficient reagents are provided to perform 100 bisulfite reactions (1 µg of DNA each*).

Kit Components: The Kit contains DNA Modification Reagents I - IV.

Cat# Description Size
S7820-KIT   CpGenome™ DNA
                   Modification Kit                                                               100 assays

 

 

CpGenome™ Universal Methylated DNA
CpGenome™ Universal Methylated DNA is enzymatically
methylated human male genomic DNA. This product is
intended for use with the CpGenome™ DNA Modification Kit (S7820-KIT) or with amplification primers. It can be used as a methylation-positive control for gene methylation studies.

Each vial contains 10 micrograms (100 µl) of human genomic DNA at a concentration of 0.1 µg/µl.

Cat# Description Size
S7821      Universal Methylated DNA            10 µg

 

 

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