Klira Rapid Ligation Kit
This rapid ligation kit enables ligation of DNA fragments with cohesive or blunt ends in less than 1 hour at room temperature. The specially formulated Klira buffer has been designed to speed up formation of phosphodiester bonds for both cohesive and blunt-end ligation. In addition, higher ligation efficiencies, as determined by transformation efficiencies, are obtained using the Klira buffer compared to using a standard ligation buffer (See Table).
This kit can be used for linker ligation, cloning in plasmid vectors, and cloning PCR products.
The Klira Kit contains sufficient reagents for 40 to 45 ligations. All reagents are guaranteed to be DNase-free.