Purification of nuclear chromosomal DNA from plants

The Floraclean Kit is a modification of the popular GNOME^® DNA Isolation Kit that has been designed specifically for isolation of nuclear chromosomal DNA from plant tissues. Purified nuclei are lysed to obtain DNA free of phenolics, polysaccharides, and othehr contaminants that can inhibit subsequent enzymatic manipulation of the DNA and that are often difficult to eliminate from DNA preps of plant cells. Noorganic extractions are required. Using this kit, up to 400 µg of DNA is isolated from 1.5g of tissue. Isolated DNA is suitable for PCR or enzymatic manipulations.

Kit Components: Nuclear Buffer Conentrate, CHO powder, Nuclear Buffer Chaotrope Solution, Nuclear Suspension Solution, Nuclear Lysis Solution, Protease Mixx, "Salt-Out" Mixture, Sieve Material