Patent-pending Peptide Nucleic Acid (PNA) Technology offers highly efficient and specific capture of mRNA

Reference Library     mTRAP™ Maxi Protocol     mTRAP™ Midi Protocol     mTRAP™ Total Protocol     Profile   Achieve higher yields than with oligo dT Eliminate rRNA and protein contamination Reduce or completely remove traces of genomic DNA Purify more representative mRNA populations Easily isolate mRNA with complex secondary structure Capture mRNA with short poly A tails Precipitate small quantities (1 µg) of mRNA without carriers or freezing

Capture mRNA with the high-affinity Poly T gripNAT Probe instead of using oligo dT.

PNAs are DNA analogs in which the four nucleotides are attached to a backbone different from the familiar deoxyribose phosphate backbone found in DNA. This remarkable chemistry allows PNAs to hybridize to complementary DNA and RNA sequences with significantly higher affinities than those of corresponding DNA oligonucleotides (oligo dT). The Poly T gripNA Probe found in the mTRAP™ mRNA Isolation Kits is a patent-pending hybrid PNA molecule offering distinct advantages over oligo dT-based purification of mRNA.

Poly T gripNA Probe has a Higher Binding Affinity than Oligo dT

• mRNA isolation can be performed in buffers with lower salt concentrations.
  • Eliminate secondary structure that can make mRNA inaccessible for capture.
  • Eliminate non-specific bonds that can cause contamination with rRNA and protein.
• mRNA is selectively eluted away from unwanted genomic DNA
• mRNA with short (< 20 bases) poly A tails are more available for capture

Poly T gripNA Probe Not Susceptible to Enzymatic Digestion like Oligo dT

• Optional DNase step can be performed if absolute control of genomic DNA is required for downstream applications

mTRAP™ Kits are available in two sizes (Midi and Maxi) for purification of mRNA directly from tissue or cells, and in one size for the purification of mRNA from total RNA. All kits include required plasticware and RNase-free solutions.

Kit Components:

The mTRAP™ Midi and Maxi Kits come with the following items: Lysis Buffer, Wash Buffer, Protease, DEPC Water, HPLC Water, Poly T gripNA™ Probe, Streptavidin Beads, 1.7 ml microcentrifuge tubes, TouchDown™ Precipitation Reagent.

The mTRAP™ Total Kit comes with the following items: 2X Binding Buffer, Wash Buffer, DEPC Water, HPLC Water, Poly T gripNA™ Probe, Streptavidin Beads, 1.7 ml microcentrifuge tubes, TouchDown™ Precipitation Reagent.

Jump to the FastPrep^® User Resource Center for information on sample lysis

Back to RNA Purification

Home | Corporate Info | Products | Services | Literature | Technical Resources

Contact | Search | Webmaster

Copyright 2002 Qbiogene, Inc. All Rights Reserved