RNA-PLUS

ready-to-use solution rapid RNA isolation green dye facilitates phase visualization suitable for all tissues and cells

Reference Library Protocol

RNA-PLUS™ enables RNA isolation from tissues or cells of human, animal, plant, yeast or bacterial origin. RNA-PLUS™ represents an improvement on Chomczynski's procedure for single step RNA isolation providing higher efficiency removal of DNA and proteins. The method promotes formation of complexes of RNA with guanidinium and water molecules and abolishes hydrophilic interactions of DNA and proteins during sample extraction. RNAs obtained with RNA-PLUS™ are not degraded and are free of proteins and DNA. The RNA is suitable for all downstream applications including hybridization, northern, polyA mRNA isolation, in vitro transcription. 1 ml of reagent is sufficient for 50µg tissue or 5 x 10⁶ cells.

Storage
Store at 4°C, do not freeze

Quality Control
All batches are performance tested

Handling
Contains guanidium thiocyanate and phenol. Wear gloves, avoid contact with skin and eyes, avoid breathing vapour. Use only phenol-resistant tubes.

References
"Single Step Method of RNA Isolation by Acid Guanidium Thiocyanate-Phenol-Chloroform Extraction." P. Chomczynski, N. Sacchi (1987), Anal. Biochem 162, 156.