is a protein which specifically inhibits ribonucleases. It is used in
applications such as in vitro translation, cDNA synthesis, RNA in vitro
synthesis, RNA purifications, etc. RNase inhibitor is easier to use and
to eliminate than vanadylribonucleosides.
One unit is the amount of protein required to inhibit the activity of
5 µg of ribonuclease A by 50 %. A minimal concentration of DTT (1
mM) is required to maintain the activity. If less than 1 mM DTT is used,the
RNase inhibitor is irreversibly inactivated. The protein is denatured
Each batch checked for absence of RNases, nickases, exo- and endonucleases.
Store at -20°C
Blackburn P (1979), J.Biol.Chem., 254, 12484.