RNase Inhibitor

RNase inhibitor is a protein which specifically inhibits ribonucleases. It is used in applications such as in vitro translation, cDNA synthesis, RNA in vitro synthesis, RNA purifications, etc. RNase inhibitor is easier to use and to eliminate than vanadylribonucleosides.

Source
human placenta

Unit Definition
One unit is the amount of protein required to inhibit the activity of 5 µg of ribonuclease A by 50 %. A minimal concentration of DTT (1 mM) is required to maintain the activity. If less than 1 mM DTT is used,the RNase inhibitor is irreversibly inactivated. The protein is denatured over 65C.

Quality Control
Each batch checked for absence of RNases, nickases, exo- and endonucleases.

Storage
Store at -20°C

References
Blackburn P (1979), J.Biol.Chem., 254, 12484.