Transform
& Grow
Bacterial Transformation Kit
- No
Heat Shock Required
- Transform-It™
Solution is Ready-to-Use
- Transform
Plasmids, Ligations, and Cosmids
- Store
Competent Cells 10 Weeks or Longer
- More
Reliable than “Do-it-Yourself” Methods
- Easy
Procedure with Minimal Hands-On Time
The
Transform & Grow™ Bacterial Transformation Kit provides
a simple and efficient method for the preparation, transformation
and storage of competent E. coli cells. Bacterial cells
are grown to mid-log phase at 37°C, pelleted and resuspended
Transform-It™ Solution. This unique buffer enables the cells
to become immediately available for transformation and permits freezing
the cells for future use without further preparation. The competent
cells remain stable at -70°C for at least 10 weeks. The Transform
& Grow™ Bacterial Transformation Kit eliminates the requirement
for heat shock and prepares competent cells within two and a half
hours with only 30 minutes of hands-on manipulation. The kit includes
Transform-It™ Solution, SUPER-COMP Media and Roll & Grow™
Plating Beads to prepare competent cells with a reliable and reproducible
transformation efficiency of 2 – 8 x106 transformants
per µg of supercoiled plasmid DNA. Reagents for 50 transformations
are provided. |
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