HER-2/ neu(ERBB2)/Alpha Satellite 17
Alterations of the HER-2/neu proto-oncogene have been implicated in the carcinogenesis and prognosis of breast cancer. The HER-2/neu (also called ERBB2) gene encodes a 185 kDa transmembrane cell surface glycoprotein and is a member of the tyrosine kinase family with a high degree of homology to the epidermal growth factor (EGF-R).

Amplification of HER-2/neu has also been reported in prostate carcinoma, uterus endometrial cancer and primary gastric cancer.

The Qbiogene probe for HER-2/neu uses the Universal Linkage System (ULS^®) and is optimized to detect amplification of the HER-2/neu gene region. The included Chromosome 17 Alpha Satellite probe serves as an internal control and simultaneously defines the ploidy status of Chromosome 17.

These direct labeled probes have been tested and qualified on cultured cells, blood smears and are optimized for formalinfixed paraffin-embedded tissue sections.

Technical Information:
The 17p12 (HER-2/neu) specific DNA Probe is direct-labeled with Rhodamine (Ex max. 565 nm; Em. max. 590 nm), the Chromosome 17 Alpha-Satellite Probe is direct-labeled with Fluorescein (Ex Max. 495 nm; Em max. 525 nm).

Localization:
Locus: 17q11.2 – q12
Gene: HER-2/neu (ERBB2)
Probe Size : 450 kb