displayPROFILE™ Kits

Convenient and reproducible technology for TOTAL Expression Profiling

• Reproducible gene expression profiles
• Total expression profiling
• Works with both eukaryotic and prokaryotic organisms
• Isotopic or fluorescent detection

displayPROFILE™ Technology
displayProfile™ technology represents a breakthrough improvement on the classic differential display technique, overcoming the original problems of reproducibility and false
positives. displayPROFILE™ technology offers the most convenient, reliable and economical solution for TOTAL expression profiling - which is impossible using microarray techniques. A novel PCR approach called RFDD-PCR (restriction fragment differential display-PCR) provides the basis for the displayPROFILE™ technique. RFDD-PCR is the amplification of double strand cDNA fragments obtained after reverse transcription, digestion by Taq I restriction enzymes and ligation to special adaptors. The adaptors permit the use of specific primers for amplification and subsequent high stringency PCR, thus making displayPROFILE™ analysis highly reliable.

Emphasis on Coding Regions
With standard differential display techniques, downstream PCR priming uses the sequence derived from the RNA poly-A tail, so a high percentage of differentially expressed sequences identified with these procedures correspond to the 3’-untranslated region. This bias can often make it difficult to compare and/or catalog results with known sequences available in various genetic databases. With displayPROFILE™, doublestranded cDNA is generated and digested with the Taq I restriction enzyme, which preferentially cuts coding regions in many organisms due to codon bias.

Predict and Identify Genes in a Profile
The displayPROFILE™ technology revolutionizes the analysis of expression profiles; indeed with a few clicks of the mouse at the displayFIT™ website: www.qbiogene.com/displayfit/, a continually
updated sequence database, the position of a band is entered and a list of sequences is generated showing all the potential genes from the database that might yield this particular fragment. This can help decide which fragments have the best potential for follow-up. Also, by using the sequence information of known genes it is possible to predict where they will appear in the profile, providing possible internal controls in your experiments.

Used in Both Eukaryotic and Prokaryotic Systems
Since displayPROFILE™ technology does not use poly-Aprimed PCR amplification, it can be used both in eukaryotic and prokaryotic systems.

Choice of Isotopic or Fluorescent Label
The displayPROFILE™ probe kits are supplied in a format suitable for isotopic detection where the O-extension primer is labeled by the end user with 33P. Also, two fluorescent versions exist: one with the O-extension primer labeled for blue fluorescence detection and another for red.