The EZ-Yeast Transformation kit is designed for high throughput transformation with an efficiency of up to 1000 colonies per µg plasmid DNA. For high efficiency transformation use the Alkali Cation Yeast Transformation Kit or the Yeast Spheroplast Transformation kit.
Transformation efficiency at different temperatures
two-hybrid reporter strain SFY526 is transformed as per protocol with
incubation at the indicated conditions. The number of transformants after
3 days at 30°C is shown. Bait, library and bait library transformants
are plated on SDA-TRP (cat.# 4812-), SDA-LEU-TRP (cat.# 4811-) and SD-LEU-TRP
(cat.# 4823-) respectively.
The various yeast two-hybrid reporter strains are transformed simultaneously with bait and library vectors as per protocol (30 min at 42°C). The number of transformats after 3 days at 30°C is shown. Bait and library vectors for SFY526, HF7C and Y190 are pVA3 and pTD1; cotransformants are plated on SD-TRP-LEU (cat.# 4823-). Bait and library for EGY48 are pEG202-P53 and pJG4-5-LTA; cotransformants are plated on SD-HIS-TRP (cat.# 4826-).
The Protocol, in brief: