sgGFP™ and sgBFP™ Vectors

High level expression vectors

A variety of sgGFP™/sgBFP™ encoding vectors are available,
optimally designed for numerous applications. CMV promoter-
driven AFP^®s are the choice for optimal transient
expression in mammalian cells as well as for the generation of AFP^®-fusion constructs or promoter evaluation. AFP^®-
neomycin fusions driven by either murine pgk or pol II promoters offer dual selection possibilities for the rapid evaluation of transfection efficiencies and for establishing stably transformed fluorescent cell-lines. In addition, Qbiogene offers localization vectors that express fusion proteins confined to various subcellular compartments.

All our vectors are designed to feature the following:

• AFPs^® with mutations that optimize protein folding and fluorophore formation at 37°C
• Codon-optimized coding sequences to stabilize mRNA structure and enhance expression without restricting host range

Stable Transformation and Transgenic Mammalian
Expression Vectors
These basic reporter vectors containing AFP^®-neomycin fusions under the control of the constitutive murine pol II or pgk promoters, are particularly well adapted for the generation of long-term stable transformants and transgenic animals. Transformants can be detected by autofluorescence, by FACS or resistance to the antibiotic G418. These vectors exist in GFP version only.

Mammalian High Level Expression Vectors
These vectors use the powerful mammalian CMV-IE promoter. Both contain an Nhe I restriction site for the generation of Nterminal fusion proteins and an SV40 promoter-neomycin selection cassette.