Mammalian sgGFP™ and sgBFP™ Cloning Vectors
In order to facilitate the use of sgGFP™ and sgBFP™ as fluorescent
tags, a wide variety of fusion vectors are available.
The pQBI25/50-fPA vectors are designed to allow easy generation
of protein fusions with Qbiogene's AFP®s and any coding
sequence cloned by PCR. Single, unique restriction sites are
located at the N-terminus (Nhe I) and C-terminus of the GFP
/BFP coding sequences. The vectors contain the powerful
CMV-IE promoter-enhancer driving the expression of
sgGFP™/ sgBFP™ with optimized initiation codon. The vectors
have been designed as a functional cassette, allowing the
isolation of the promoter region, GFP/BFP coding sequences,
poly A signal sequence or any combination thereof. 
AFP2210 pQBI25-fPA 20 µg
AFP2221 pQBI25-fA1 20 µg
AFP2222 pQBI25-fA2 20 µg
AFP2223 pQBI25-fA3 20 µg
AFP2224 All three fA1 fA2 fA3 20 µg of each reading frame
AFP2231 pQBI25-fC1 20 µg
AFP2232 pQBI25-fC2 20 µg
AFP2233 pQBI25-fC3 20 µg
AFP2234 All three fC1 fC2 fC3 20 µg of each reading frame
AFP2211 pQBI25-fN1 20 µg
AFP2212 pQBI25-fN2 20 µg
AFP2213 pQBI25-fN3 20 µg
AFP2214 All three fN1 fN2 fN3 20 µg of each reading frame |
Storage: -20° C |
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All other series of vectors: pQBI25/50-fN 1,2,3, pQBI25/50-fA
1,2,3 and pQBI25/50-fC 1,2,3, have complete Multiple Cloning
Sites (MCS) in all three open reading frames, at the N- or C-terminus
of the AFP®s coding region. These vectors also have a
peptide linker placed between the AFP® and the MCS to
encourage independent folding of the two protein moities without
steric hindrance. The fA series of vectors contain “Kozak”
optimized initiation codons for optimal expression, whereas the
fN series of vectors have no initiation codon, allowing the use
of the endogenous sequences of the fusion protein. All of these
vectors have been designed as functional cassettes, permitting
the isolation of the CMV promoter region, AFP®s coding
sequence, the polyadenylation signal sequence, or any combination
thereof. Each vector also contains the SV40 neo cassette
for biochemical selection of transformed cells.
AFP2110 pQBI50-fPA 20 µg
AFP2121 pQBI50-fA1 20 µg
AFP2122 pQBI50-fA2 20 µg
AFP2123 pQBI50-fA3 20 µg
AFP2124 All three fA1 fA2 fA3 20 µg of each reading frame
AFP2131 pQBI50-fC1 20 µg
AFP2132 pQBI50-fC2 20 µg
AFP2133 pQBI50-fC3 20 µg
AFP2134 All three fC1 fC2 fC3 20 µg of each reading frame
AFP2111 pQBI50-fN1 20 µg
AFP2112 pQBI50-fN2 20 µg
AFP2113 pQBI50-fN3 20 µg
AFP2114 All three fN1 fN2 fN3 20 µg of each reading frame |
Storage: -20° C |
