Autofluorescent Proteins and Fusion Vectors

 

Vectors for Subcellular Localization of Proteins
Generate fully bi-functional, localized fusion proteins between
AFP’s and HIV-1 proteins or the human B23 protein. Several sgGFP™ and sgBFP™ localization vectors are available. These vectors, expressing a fusion protein between the sgGFP™ or sgBFP™ and HIV-1 proteins or human B23 protein, localize to various subcellular regions.
 

Sequence information

For complete sequences of these and all other vectors please see www.qbiogene.com.

Localized Green fluorescence

Cat# Vector Promoter Description Localization Size
AFP3201     pQBI-B23sgGFP     CMV                                  HumanB23-GFP       Nucleolus                                   20 µg
AFP3202     pQBI-tatGFP          CMV                                  tat-GFP                    Nucleolus/Nucleus                     20 µg
AFP3203     pQBI-nefGFP          CMV                                  nef-GFP                   Golgi, endosomes, etc.              20 µg
AFP3205     pQBI-m10-GFP       CMV                                  m10BLrev-BFP         Nucleolus                                  20 µg
AFP3206     pQBI-vprGFP          CMV                                  vpr-GFP                   Nucleolus/Nuclear membrane    20 µg
AFP3207     pQBI-LTRgagGFP    HIV LTR(rev-dependent)  gag-GFP                  Cytoplasmic (rev-dependent)    20 µg

Localized Blue fluorescence

Cat# Vector Promoter Description Localization Size
AFP3101     pQBI-B23sgBFP     CMV                                  Human B23-BFP        Nucleolus                                   20 µg
AFP3104     pQBI-revBFP          CMV                                  HIV-1 rev-BFP           Nucleolus/cytoplasm                  20 µg
AFP3114     pQBI-LTRrevBFP     HIV LTR                            HIV-1 rev-BFP           Nucleolus/cytoplasm                  20 µg
AFP3115     pQBI-LTRm10-BFP  HIV LTR                            m10BLrev-BFP          Nucleolus                                   20 µg

 

 

 

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