Bacterial Growth Media - Media Components and Plating Tools

Media Components and Plating Tools

Raw Materials for Media Formulation

Yeast Extract-B

A dried and powdered, water-soluble extract of autolysed
yeast; rich in B vitamins, amino acids and peptides.

Cat #		Size
Powder
3065-512 3065-522 3065-532 3065-542		227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb)
Capsules
3065-511 3065-521 3065-531 3065-541		227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb)

Yeast Extract-B, Granulated

This type of Yeast Extract-B is formed into small granules,
making it easier to handle than the fine powdered form.

Cat #		Size
Granulated Powder
4023-012 4023-022 4023-032 4023-042		227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb)

Tryptone-B

Tryptone-B is a dried and powdered pancreatic digest of casein. The bacteriological grade has been chosen for optimum growth of bacteria.

Cat #		Size
Powder
3066-012 3066-022 3066-032 3066-042		227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb)
Capsules
3066-011 3066-021 3066-031 3066-041		227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb)

Casein Peptone

Casein Peptone, Type I.

Cat #		Size
Powder
3066-112 3066-122 3066-132 3066-142		227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb)

Casamino Acids

Acid hydrolyzed casein; rich in amino acids except tryptophan.

Cat #		Size
Powder
3060-012 3060-022 3060-032 3060-042		227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb)
Capsules
3060-011 3060-021 3060-031 3060-041		227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb)

Agar-B

Bacteriological grade Agar-B demonstrates a superior ability to promote bacterial colony growth (colony diameter after 24 and 48 hours) and phage growth (plaque diameter and morphology of both M13 and Lambda bacteriophages). Agar-B is supplied as a light, tan-colored, small granular form.

Cat #		Size
Powder
3072-012 3072-022 3072-032 3072-042		227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb)
Pouches
3072-065 3072-085 3072-075 3072-095		10 x 0.5 L Pouches 100 x 0.5 L Pouches 10 x 1.0 L Pouches 100 x 1.0 L Pouches
Capsules
3072-011 3072-021 3072-031		227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb)

Noble Agar

Nutrient-free agar used in defined media formulations where traces of nutrients cannot be tolerated.

Cat #		Size
Powder
3073-012 3073-022 3073-032 3073-042		227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb)

Carbon Sources/Sugars

Cat #	Description	Size
4021-002 4008-012 4008-022 4008-032 4008-034 3055-012 3055-022 3055-032 3055-042 3055-534 3055-544 4006-012 4006-022 4006-042 3068-034 3068-044 4061-512 4061-522 4010-012 4010-022 4010-032 4010-034 4013-012 4013-022 4013-032 4020-032 4020-042	D-Galactose, Ultra Pure (Powder) D-Galactose, Glucose-Free (0.1% glucose) D-Galactose, Glucose-Free (0.1% glucose) D-Galactose, Glucose-Free (0.1% glucose) Sterile 20% Galactose, Glucose-Free (0.1% glucose) Glucose (Dextrose) Glucose (Dextrose) Glucose (Dextrose) Glucose (Dextrose) Sterile 20% Glucose Sterile 20% Glucose Maltose Maltose Maltose Sterile 20% Maltose Sterile 20% Maltose D( ) Raffinose Pentahydrate D( ) Raffinose Pentahydrate D( ) Raffinose Pentahydrate D( ) Raffinose Pentahydrate D( ) Raffinose Pentahydrate Sterile 20% Raffinose Sucrose Sucrose Sucrose D-Sorbitol, Ultra Pure D-Sorbitol, Ultra Pure	25.0 g 227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 500 ml 227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb) 100 ml 500 ml 227 g (0.5 lb) 454 g (1 lb) 5 kg (11 lb) 100 ml 500 ml 10 g 100 g 227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 500 ml 227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 1 kg (2.2 lb) 5 kg (11 lb)

Antibiotics

Cat #		Size
Ampicillin, Sodium Salt
2610-212 AMP10010 2610-222		5.0 g 10 x 1.0 g 50 g
Kanamycin Sulfate
2710-202 2710-212 2710-222		1.0 g 5.0 g 25.0 g
Carbenicillin Sodium
2711-202 2711-212		1.0 g 5.0 g
Tetracycline Hydrochloride
2720-202 2720-212		25.0 g 100 g
Chloramphenicol
2725-201		100 g
G418 Sulfate
4067-102 4067-112		1.0 g 5.0 g

Other Supplements

X-GAL

5-bromo-4-chloro-3-indolyl-b-D-galactosidase, Ultra-Pure.

Cat #		Size
4063-102 4063-112 XGAL0100 XGAL0500 XGAL1000 XGAL5000		1.0 g 5.0 g 100 mg 5 x 100 mg 1.0 g 5 x 1.0 g

XTRA-Blue™ Plus X-Gal/IPTG Solution

Pour your plates in advance saving time and effort

Ready-to-use X-Gal/IPTG solution
Stable at 4° C
Stable when plated with agar and antibiotics
Non-toxic solution (no DMF, no DMSO)
For blue/white screening in bacteria and mammalian cells

With Qbiogene’s XTRA-Blue™ Plus X-Gal/IPTG solution no
DMF/DMSO is required. XTRA-Blue™ Plus is a ready-made
solution containing X-Gal and IPTG that maintains its functional activity for months at 4°C. Due to its high stability XTRA-Blue™ Plus can be mixed with agar and antibiotics and poured in plates in advance of requirements. For the user this means a valuable saving in time and effort.

XTRA-Blue™ Plus solution contains non-hazardous, non-toxic solvents and so is also suitable for use with cultured mammalian cells.

Storage at 4°C avoids the freeze-thaw cycles that can impair the quality of X-Gal and IPTG leading to greater reliability of results.

Another remarkable advantage of XTRA-Blue™ Plus is its efficiency as a substrate - no incubation at 4°C is needed to generate a bright blue coloration for "negative" colonies.

Quality control
Each batch is tested against classical X-Gal/IPTG method, with 2 different strains expressing pUC19 (high copy plasmid) or BAC2 (low copy plasmid).

Sterility
XTRA-Blue™ Plus is filtered through 0.22 micron membrane.

Cat #	Description	Size
XBLUP010 XBLUP100	XTRA-Blue™ Plus solution XTRA-Blue™ Plus solution	10 ml (100 plates) 100 ml (1000 plates)

IPTG

Isopropylthio-b-galactosidase, Ultra-Pure.

Cat #		Size
4064-102 4064-112 IPTG0001 IPTG0005 IPTG0010		1.0 g 5.0 g 1.0 g 5.0 g 10 x 1.0 g

Sterile 80% Glycerol

Easy to pipet; excellent for freezing strains.

Cat #		Size
3055-024 3055-034 3055-044		50 ml 100 ml 500 ml

Plating Tools

Roll & Grow^® Plating Beads

Safely and easily spread yeast and bacterial cells

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Spread cells in just seconds per plate
Eliminate hazards of flame and ethanol
No need for fragile glass “hockey sticks”
Consistent, even colony distribution
Sterile and easy to use

Roll & Grow^® Plating Beads are used to spread 25-400 µl of
bacterial or yeast cells across the surface of an agar plate, providing a safer, faster alternative to the traditional method of plating with a glass rod sterilized with ethanol and flame. Sterile Roll & Grow^® Plating Beads are provided in two skirted 50 ml conical tubes. Between six and seven 4 mm glass beads are used per plate for a total of approximately 160 standard 100 mm plates.

Cat #	Description	Size
5000-550	Roll & Grow^® Plating Beads, 2 Pack	2 tubes

Replica Plating Apparatus

Made from hardwood, the Replica Plating Apparatus can be used with velvet, gauze, or even paper towels to transfer colonies from plate to plate or plate to filter while retaining colony orientation. The Replica Plating Apparatus and Velvet Squares can be ordered to fit either 100 mm or 150 mm plates.

Cat # Description Size

5000-001
5000-006
5000-007
5000-004
5000-008
5000-009
Replica Plating Apparatus
Velvet Pad, 6 Inch Square
Velvet Pad, 6 Inch Square (Pack of 10)
Replica Plating Apparatus
Velvet Pad, 9 Inch Square
Velvet Pad, 9 Inch Square (Pack of 10) 100 mm
100 mm
100 mm
150 mm
150 mm
150 mm

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