CIRCLEGROW® Broth and Agar
Extend plasmid production past log phase
CIRCLEGROW® media is designed for obtaining rapid growth of E. coli and high yields of plasmid DNA. Bacteria grown in conventional culture media reach an abrupt stationary phase upon exhaustion of nutrients. Cell proliferation is halted completely and some cell death and plasmid loss occurs.
At the end of log phase, cells growing in CIRCLEGROW® begin to utilize a proprietary second carbon source which becomes available on a delayed basis. Cell growth and plasmid replication continue long after cells in conventional media have reached stationary phase or expired. Bacteria commonly grow in CIRCLEGROW® to a density triple that achieved in other media (see figure). The slow post-log phase growth of bacteria in CIRCLEGROW® eliminates the need for constant OD monitoring. CIRCLEGROW® cultures left overnight or over the weekend will have a much lower percentage of dead cells, and a higher percentage of plasmid DNA, than cultures prepared with any other media. The result is a higher yield of plasmid DNA in less volume with less attention.
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CIRCLEGROW® Broth
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40 g (40 Capsules, 8 Large Capsules) per Liter
Contents/L: Proprietary Formulation |
| Powder |
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3000-112
3000-122
3000-132
3000-142
3000-152 |
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227 g (0.5 lb)
454 g (1 lb)
908 g (2.0 lb)
2.3 kg (5.0 lb)
5 kg (11 lb) |
| Pouches |
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3000-165
3000-185
3000-175
3000-195 |
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10 x 0.5 L Pouches
100 x 0.5 L Pouches
10 x 1.0 L Pouches
100 x 1.0 L Pouches |
Capsules
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3000-121
3000-131
3000-141 |
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250 g (250 Caps)
1 kg (1,000 Caps)
2.5 kg (2,500 Caps) |
Large Capsules
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3000-116
3000-126
3000-136
3000-146 |
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227 g (0.5 lb)
454 g (1 lb)
1 kg (2.2 lb)
5 kg (11 lb) |
Sterile Solution
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| 3000-104 |
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500 ml |
CIRCLEGROW® Agar
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55 g (11 Large Capsules) per Liter
Contents/L: Proprietary Formulation |
| Powder |
3100-112
3100-122
3100-132
3100-142 |
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227 g (0.5 lb)
454 g (1 lb)
1 kg (2.2 lb)
2.3 kg (5.0 lb) |
| Large Capsules |
3100-116
3100-126
3100-136
3100-146 |
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227 g (0.5 lb)
454 g (1 lb)
1 kg (2.2 lb)
5 kg (11lb) |
Pre-Poured Plates (no antibiotic)
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| 3100-124 |
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10 x 100mm Plates |
| 3100-134 |
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100x 100mm Plates |
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GS96 Broth for Multi-well Plates
High yields of plasmid DNA in 96-well and 384-well plates
GS96 Broth is formulated to grow host cells to the highest density possible without aeration, and to produce high yields of plasmid DNA from those cultures. Perfect for growing bacterial cells in 96-well and 384-well plates, GS96 media can be used in any situation in which aeration of the culture is difficult or absent. GS96 broth is used at the front end of highthroughput automated procedures, and growth can be increased even further by the addition of 0.05% glycerol. |
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GS96 Broth
50 g per Liter
Contents/L: Proprietary Formulation
| Powder |
3101-112
3101-122
3101-132
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227 g (0.5 lb)
454 g (1 lb)
1 kg (2.2 lb) |
| Pouches |
3101-065
3101-085
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10 x 0.5 L Pouches
100 x 0.5 L Pouches |
Sterile Solution
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| 3101-124 |
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500 ml |
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Plasmid Growth Formulations (PGF)
Optimize plasmid yield in a given host cell
Each individual plasmid growth formulation (PGF) demonstrates different growth characteristics for a given plasmid/host system. Use the PGF test pack to determine which of the five different PGF formulations work best for a specific plasmid/host combination, then order the individual PGF formulation that works best for you! |
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Plasmid Growth Formulations
PGF-843: 45 g per Liter; PGF-6277: 50 g per Liter; PGF-426: 40 g per Liter; PGF-1287: 52 g per Liter; PGF 1121: 39 g per Liter
Contents/L: Proprietary Formulation
| Powder |
3302-312
3302-712
3302-412
3302-512
3302-612 |
PGF-843
PGF-1121
PGF-6277
PGF-426
PGF-1287
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227 g (0.5 lb)
227 g (0.5 lb)
227 g (0.5 lb)
227 g (0.5 lb)
227 g (0.5 lb) |
| Pouches |
3302-115
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PGF Test Pack |
10 x 1.0 L pouches, 2 each |
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Nutrient Agar Stabs
Contents/liter: Proprietary LB-like formulation containing glucose, cysteine and thymine.
Stabs are a convenient way to store or transport bacterial strains. Vials of soft nutrient agar are stabbed with a loop of a fresh culture and incubated overnight. The caps are then tightened
and the cultures are stored in the dark. Special glass vials ensure an airtight environment for at least two years, and most cell types will remain viable for at least this long. |
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Nutrient Agar Stabs
| Powder |
3050-023
3050-033
3050-043
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25 Stabs
50 Stabs
100 Stabs |
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