Standard E. coli Growth Media


LB Medium Recipes

Recipes for the E. coli growth media known as LB Medium or L-Broth typically contain 10 g of tryptone and 5 g of yeast extract per liter, and can vary in salt concentration from 0.5 g to 10 g per liter. BIO 101® Systems offers three forms of this traditional media: LB Medium and LB Agar contain 10 g NaCl per liter, LB Medium, Lennox and LB Agar, Lennox contain 5 g of NaCl per liter, and L-Broth and L-Broth Agar contain 0.5 g NaCl per liter. A NaCl-free version of LB Medium is also available for use in salt-inducible recombinant protein expression.

 

LB Medium 25 g (25 Capsules or 4 Large Capsules) per liter Contents/liter: 10 g tryptone, 5 g yeast extract, 10 g NaCl Cat #   Size Powder 3002-092 3002-012 3002-022 3002-032 3002-042   125 g 227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb) Pouches 3002-065 3002-085 3002-075 3002-095   10 x 0.5 L Pouches 100 x 0.5 L Pouches 10 x 1.0 L Pouches 100 x 1.0 L Pouches Capsules 3002-011 3002-021 3002-031 3002-041   227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb) Large Capsules 3002-016 3002-026 3002-036 3002-046   227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb) Sterile Solution 3002-014 3002-024   500 ml 1.0 L         LB-Agar Medium 40 g (40 Capsules or 8 Large Capsules) per liter Contents/liter: LB Medium, 15 g Agar-B Cat #   Size Powder 3002-262 3002-212 3002-222 3002-232 3002-242   120 g 227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb) Pouches 3002-265 3002-285 3002-275 3002-295   10 x 0.5 L Pouches 100 x 0.5 L Pouches 10 x 1.0 L Pouches 100 x 1.0 L Pouches Capsules 3002-211 3002-221 3002-231 3002-241   227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb) Large Capsules 3002-216 3002-226 3002-236 3002-246   227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb) Sterile Solution 3002-204   500 ml Pre-Poured Plates (no additives) 3002-224 3002-234   10 x 100mm plates 100 x 100mm plates Pre-Poured Plates (w/ 50 µg/ml Ampicillin) 3014-124 3014-134   10 x 100mm plates 100 x 100mm plates Pre-Poured Plates (w/ 100 µg/ml Ampicillin) 3014-224 3014-234   10 x 100mm plates 100 x 100mm plates Pre-Poured Plates (w/ 30 µg/ml Kanamycin) 3015-224 3015-234   10 x 100mm plates 100 x 100mm plates Pre-Poured Plates (w/ 50 µg/ml Kanamycin) 3015-324 3015-334   10 x 100mm plates 100 x 100mm plates Pre-Poured Plates (w/ 100 µg/ml Carbenicillin) 3019-124 3019-134   10 x 100mm plates 100 x 100mm plates Pre-Poured Plates (w/ 100 µg/ml Ampicillin, 40 µg/ml X-GAL & 0.5 mM IPTG) 3017-124 3017-134   10 x 100mm plates 100 x 100mm plates         LB-Top Agar 32 g (32 Capsules or 6 Large Capsules) per liter Contents: LB Medium, 7 g Agar-B Cat #   Size Powder 3002-512 3002-522   227 g (0.5 lb) 454 g (1 lb) Capsules 3002-511 3002-521   227 g (0.5 lb) 454 g (1 lb)         LB Medium, Lennox 20 g powder (20 Capsules or 4 Large Capsules) per Liter Contents: 10 g tryptone, 5 g yeast extract, 5 g NaCl Cat #   Size Powder 3002-112 3002-122 3002-132 3002-142   227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb) Pouches 3002-165 3002-185 3002-175 3002-195   10 x 0.5 L Pouches 100 x 0.5 L Pouches 10 x 1.0 L Pouches 100 x 1.0 L Pouches Capsules 3002-111 3002-121 3002-131 3002-141   227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb) Large Capsules 3002-116 3002-126 3002-136 3002-146   227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb) Sterile Solution 3002-104   500 ml         LB Agar, Lennox 35 g (35 Capsules) per Liter Contents: LB Medium, Lennox, 15g/L Agar-B Cat #   Size Powder 3002-312 3002-322 3002-332   227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) Pouches 3002-365 3002-385 3002-375 3002-395   10 x 0.5 L Pouches 100 x 0.5 L Pouches 10 x 1.0 L Pouches 100 x 1.0 L Pouches Capsules 3002-311 3002-321 3002-331   227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb)         L-Broth 16 g (16 Capsules) per Liter Contents/liter: 10 g tryptone, 5 g yeast extract, 0.5 g NaCl Cat #   Size Powder 3001-012 3001-022 3001-032 3001-042   227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb) Pouches 3001-065 3001-085 3001-075 3001-095   10 x 0.5 L Pouches 100 x 0.5 L Pouches 10 x 1.0 L Pouches 100 x 1.0 L Pouches Capsules 3001-011 3001-021 3001-031 3001-041   227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb)         L-Broth Agar 31 g (31 Capsules) per Liter Contents/liter: L-Broth, 15 g Agar-B Cat #   Size Powder 3001-212 3001-222 3001-232 3001-242   227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb) Pouches 3001-265 3001-285 3001-275 3001-295   10 x 0.5 L Pouches 100 x 0.5 L Pouches 10 x 1.0 L Pouches 100 x 1.0 L Pouches Capsules 3001-211 3001-221 3001-231 3001-241   227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb) Large Capsules 3001-216 3001-226 3001-236 3001-246   227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb)         L-Broth Top Agar 23 g (23 Capsules) per Liter Contents/liter: L-Broth, 7 g Agar-B Cat #   Size Powder 3001-512 3001-522   227 g (0.5 lb) 454 g (1 lb) Capsules 3001-511 3001-521   227 g (0.5 lb) 454 g (1 lb)         LB Medium w/o Sodium Chloride 15 g per Liter Contents/liter: 10 g tryptone, 5 g yeast extract Cat #   Size Powder 30202-012 30202-022 30202-032   227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb)