Bacterial Growth Media - Standard E. coli Growth Media

Standard E. coli Growth Media

Rich and Minimal Formulations

Terrific Broth

Part 1: 36 g (36 Capsules) per Liter
Part 2: Potassium phosphate salts and glycerol in sterile solution
Contents/liter: 24 g yeast extract, 12 g tryptone

Cat #		Size
Powder, Part 1 (includes Part 2 sterile liquid)
3045-012 3045-022 3045-032 3045-042		227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb)
Capsules, Part 1 (includes Part 2 sterile liquid)
3045-011 3045-021 3045-031		227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb)

Terrific Broth II

50 g (50 Capsules) per Liter
Contents/L: Proprietary Formulation

Terrific Broth II is a proprietary formulation that is shipped as a single powder instead of as two separate components. Terrific Broth II exhibits the same growth characteristics as the original
Terrific Broth formula.

Cat #		Size
Powder
3046-012 3046-022 3046-032 3046-042		227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb)
Pouches
3046-065 3046-085 3046-075 3046-095		10 x 0.5 L Pouches 100 x 0.5 L Pouches 10 x 1.0 L Pouches 100 x 1.0 L Pouches
Capsules
3046-011 3046-021 3046-031 3046-041		227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb)

SuperBroth

60 g (60 Capsules or 12 Large Capsules) per Liter
Contents/liter: 35 g tryptone, 20 g yeast extract, 5 g NaCl

Cat #		Size
Powder
3010-012 3010-022 3010-032 3010-042		227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb)
Pouches
3010-065 3010-085 3010-075 3010-095		10 x 0.5 L Pouches 100 x 0.5 L Pouches 10 x 1.0 L Pouches 100 x 1.0 L Pouches
Capsules
3010-011 3010-021 3010-031 3010-041		227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb)

Large Capsules
3010-016 3010-026 3010-036 3010-046		227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb)
Sterile Solution
3010-014 3010-024		500 ml 1.0 L

SuperBroth Agar

75 g (75 Capsules) per Liter
Contents/liter: SuperBroth, 15 g Agar-B

Cat #		Size
Powder
3010-212 3010-222 3010-232 3010-242		227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb)
Capsules
3010-211 3010-221 3010-231 3010-241		227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb)

SuperBroth Top Agar

67 g (67 Capsules) per Liter
Contents/liter: SuperBroth, 7 g Agar-B

Cat #		Size
Powder
3010-512 3010-522 3010-532		227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb)
Capsules
3010-511 3010-521 3010-531		227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb)

Tryptone Broth

18 g (18 Capsules) per Liter
Contents: 10 g tryptone-B, 8 g NaCl

Cat #		Size
Powder
3003-012 3003-022 3003-032 3003-042		227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb)
Capsules
3003-011 3003-021 3003-031 3003-041		227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb)

Tryptone Agar

33 g (33 Capsules) per Liter
Contents: Tryptone Broth, 15 g Agar-B

Cat #		Size
Powder
3003-212 3003-222 3003-232 3003-242		227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb)
Capsules
3003-211 3003-221 3003-231 3003-241		227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb)

Tryptone Top Agar

25 g (25 Capsules) per Liter
Contents/liter: Tryptone Broth, 7 g Agar-B

Cat #		Size
Powder
3003-512 3003-522		227 g (0.5 lb) 454 g (1 lb)
Capsules
3003-511 3003-521		227 g (0.5 lb) 454 g (1 lb)

M9 Minimal Medium Salts

Part A (g/L of 50x): 350 g K₂HPO₄, 100 g KH₂HPO₄
Part B (g/L of 50x): 29.4 g sodium citrate, 50 g (NH₄)2SO₄, 5 g MgSO₄

Parts A and B are initially dissolved in water, autoclaved, and thereafter serve as 50x concentrated stock solutions for making M9 Growth Media. To make M9 Growth Media, 2% parts A and
B are added to sterile water (or water plus melted, purified agar). Additional nutrients, such as glucose, casamino acids, vitamins, or antibiotics are added when the solution cools and before plates are poured or media is inoculated with bacteria.

Cat #		Size
Powder for 200 ml 50x M9 Salts (includes Part B, makes 10 L total)
3035-012		90 g
Powder for 1000 ml 50x M9 Salts (includes Part B, makes 50 L total)
3035-022		450 g

M9 Minimal Medium Salts

(1-part Maniatis formulation)
Contents/L of 5x solution: 33.9 g Na₂HPO₄, 15 g KH₂PO₄, 2.5 g NaCl, 5 g NH₄Cl

Cat #		Size
Powder
3037-012 3037-022 3037-032 3037-042		227 g (0.5 lb) 454 g (1 lb) 1 kg (2.2 lb) 5 kg (11 lb)

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