Introduction

BIO 101^® Systems is well known for providing high-quality growth media in a variety of formulations for every molecular biologist. We specialize in formulations for yeast and bacterial genetics, and offer more than a thousand recipes and variations. Each product is subjected to extensive quantitative testing, and is Molecular Biology Certified™ through qualitative, molecular biology based tests such as cell density and plasmid yield.

• Unbeatable selection of premixed formulations
• Proprietary media for enhanced cell and plasmid yield
• Convenient packaging formats for a variety of applications and budgets
• Molecular Biology Certified™ for the highest quality
• Proven and reliable reputation
• High-quality raw materials and additives
• Customized recipes and packaging

Wide variety of pre-mixed media formulations for E. coli BIO 101® Systems offers unique, specialty media for increased plasmid yield and protein expression, as well as
	all of the conventional media formulations. If you need something even more specialized, we can easily make your custom recipe from our Molecular Biology Certified™ raw materials.

Convenient packaging formats
BIO 101^® Systems is the only source for encapsulated growth media. The ultimate in ease and convenience, our patented media capsules eliminate the waste, inaccuracies, and mess associated with weighing out bulk powder. Simply drop the capsules in water and autoclave! Pre-mixed powders are also available in single-use pouches or in larger bottles and drums. Liquid media, pre-poured plates, and custom packaging options are also available.

Molecular Biology Certified™ growth media is designed and tested to meet the specific needs of a molecular biologist. In addition to the standard qualitative and quantitative assays of solubility, color, clarity, pH, and culture confluency, all bacterial culture media are tested for molecular biology applications such as plasmid extraction, DNA/RNA yield from the strain grown in the media, optimized protein expression, and reproducibility. The extracted plasmid or genomic DNA must also meet our high quality standards for PCR and restriction enzyme digestion, and purified proteins must maintain a high percentage of original activity.

The process of Molecular Biology Certification for LB Medium is outlined in the figure below. Several batches of yeast extract and tryptone are made and subjected to quantitative testing. In this example, yeast extract batches numbered “1”, “4”, and “5”, and tryptone batches lettered “b”, “d”, and “f” passed the first rounds of quality control. Small batches of LB Medium are made with every combination of these qualified ingredients and tested for plasmid yield. The best combination (yeast extract “5” with tryptone “b”) is chosen for the larger batch of media, to which NaCl is added prior to milling the powder into a consistent pre-mixed product. Upon completion, all qualitative tests are performed in addition to re-verification of the quantitative data.