From
Avian Myeloblastosis Virus
- Highly
active
 dimer
- Amplification
of RNA
- No 5' to 3'
exonuclease activity
- RNA-directed
DNA polymerase
- DNA-dependent
DNA polymerase
- RNase H and
unwinding activity
AMV
Reverse Transcriptase is an RNA dependent DNA polymerase that
catalyses the polymerisation of nucleotides using template DNA,
RNA or RNA: DNA hybrids.
AMV
Reverse Transcriptase is used chiefly to transcribe mRNA into
cDNA which can then be cloned into vectors or amplified by PCR.
It may also be used for filling in and labeling the 3'-termini of
DNA with 5'-protruding ends. AMV Reverse Transcriptase can also
be used for dideoxy DNA sequencing and RNA sequencing.
Storage
KPO4 200mM, pH7.2, DTT 2mM, Triton X-100 0.2%,
Glycerol 50% Temperature -20C
Incubation
buffer
Tris HCl 50mM, pH 8.3, KCl 40mM, MgCl2 6mM.
Unit
definition
One unit incorporates one nmol of TMP into DE-81 adsorbable form
in 10 minutes at 37C using polyA-oligo dT12-18 as a substrate.
Purity
Free of contaminating endonuclease, exonuclease and ribonuclease.
Activity
10-20 units/l. with a specific activity higher than 200 000
units per mg.
Functional
testing
When globin mRNA is incubated for 5 minutes at 37C, at least
99% of cDNA products are full length.
| EMAMV200 |
AMV reverse
transcriptase 10-20 U/l |
200 U |
| EMAMV203 |
AMV reverse
transcriptase 10-20 U/l |
600 U (3 x
200 U) |
| Supplied
with 10 x incubation buffer |
 Patent Information
for PCR Process
Related products
First Strand Synthesis Opti-Kit
|
