Arrow Taq Polymerase

Arrow Taq™ DNA Polymerase (patent pending) is a novel mixture of thermostable DNA polymerases which offers several advantages over PCR with recombinant Taq DNA polymerase alone. Its efficiency is higher and smaller quantities of template DNA are needed than with Taq alone. Efficient and specific amplification of fragments up to 21kb in length can be expected with higher fidelity than Taq DNA Polymerase.

Because Arrow Taq™ is a mixture of Taq DNA Polymerase and Tfu DNA Polymerase which has proofreading capability, it exhibits a lower error rate than Taq.

The presence of Taq means there are few constraints regarding amplification parameters which means less optimization is necessary than when a proofreading enzyme is used alone.

Maximum fidelity is achieved with 40µM of each dNTP and the buffer provided. For longer fragments the amount of dNTP must be increased to 200µM with some increase in error rates.

Incubation buffer
10mM Tris-HCl pH 9.0, 50mM KCl, 1.5mM MgCl₂, 0.1% Triton X-100, 0.2 mg/ml BSA. 10 X buffer provided.

Storage
20mM Tris-HCl pH 8.0 , 100mM KCl , 0.1mM EDTA, 1mM dithiothreitol , 0.5% Tween 20, 0.5% Nonidet P40, 50% glycerol at -20°C.

Quality control
Absence of nickases and endonucleases, absence of ribonucleases, specific PCR on genomic and phage templates.